PUBLICATION

Fgf8 drives myogenic progression of a novel lateral fast muscle fibre population in zebrafish

Authors
Groves, J.A., Hammond, C.L., and Hughes, S.M.
ID
ZDB-PUB-050831-2
Date
2005
Source
Development (Cambridge, England)   132(19): 4211-4222 (Journal)
Registered Authors
Groves, Julie, Hammond, Chrissy, Hughes, Simon M.
Keywords
Fibroblast growth factor 8, Muscle, Zebrafish, Fast, Myod, Somite
MeSH Terms
  • Animals
  • Body Patterning/physiology
  • Cell Differentiation
  • Embryo, Nonmammalian/metabolism
  • Fibroblast Growth Factor 8/physiology*
  • Hedgehog Proteins
  • Muscle Fibers, Fast-Twitch/cytology
  • Muscle Fibers, Fast-Twitch/physiology*
  • MyoD Protein/biosynthesis
  • Myoblasts/metabolism
  • Myoblasts/physiology*
  • Myogenic Regulatory Factor 5/biosynthesis
  • Myogenin/metabolism
  • Paired Box Transcription Factors/biosynthesis
  • Signal Transduction
  • Somites/physiology*
  • Trans-Activators/physiology
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Zebrafish Proteins/biosynthesis
PubMed
16120642 Full text @ Development
Abstract
Fibroblast growth factors (Fgfs) have long been implicated in regulating vertebrate skeletal muscle differentiation, but their precise role(s) in vivo remain unclear. Here, we show that Fgf8 signalling in the somite is required for myod expression and terminal differentiation of a subset of fast muscle cells in the zebrafish lateral somite. In the absence of Fgf8, lateral somite cells transiently express myf5 but fail to make muscle and remain in a dermomyotome-like state characterised by pax3 and meox expression. Slow muscle fibres form and commence normal migration in the absence of Fgf8, but fail to traverse the expanded undifferentiated lateral somite. The Fgf8-independent residual population of medial fast muscle fibres is not Hedgehog dependent. However, Fgf8-independent medial fast muscle precursors are lacking in floatinghead mutants, suggesting that they require another ventral midline-derived signal. We conclude that Fgf8 drives terminal differentiation of a specific population of lateral muscle precursor cells within the early somite.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes