PUBLICATION
Use of transgenic zebrafish reporter lines to study calcium signalling in development
- Authors
- Ashworth, R., and Brennan, C.
- ID
- ZDB-PUB-050818-14
- Date
- 2005
- Source
- Briefings in functional genomics & proteomics 4(2): 186-193 (Journal)
- Registered Authors
- Ashworth, Rachel, Brennan, Caroline
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified*
- Calcium/metabolism*
- Calcium Signaling
- Embryo, Nonmammalian/physiology
- Embryonic Development
- Fluorescence Resonance Energy Transfer/methods
- Gene Expression Regulation, Developmental*
- Genes, Reporter*
- Luminescent Proteins/chemistry
- Neurons/metabolism
- Signal Transduction
- Time Factors
- Zebrafish
- PubMed
- 16102273 Full text @ Brief. Funct. Genomic. Proteomic.
Citation
Ashworth, R., and Brennan, C. (2005) Use of transgenic zebrafish reporter lines to study calcium signalling in development. Briefings in functional genomics & proteomics. 4(2):186-193.
Abstract
Calcium signals are associated with many of the events common to animal development. Understanding the role of these calcium signals requires the ability to visualise and manipulate calcium levels in the developing embryo. Recent work has led to the development of sensitive protein-based probes that can be used to generate transgenic animals for the analysis of calcium signalling in vivo. This paper focuses on the use of genetically encoded calcium probes to follow calcium signals in zebrafish. It reviews progress and speculates on the potential for use in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping