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ZFIN ID: ZDB-PUB-050607-22
Identification of a gene required for de novo DNA methylation of the zebrafish no tail gene
Shimoda, N., Yamakoshi, K., Miyake, A., and Takeda, H.
Date: 2005
Source: Developmental dynamics : an official publication of the American Association of Anatomists   233(4): 1509-1516 (Journal)
Registered Authors: Shimoda, Nobuyoshi, Takeda, Hiroyuki
Keywords: zebrafish; no tail; methylation; methyltransferase; morpholino oligo (MO)
MeSH Terms:
  • Animals
  • Catalytic Domain/genetics
  • DNA (Cytosine-5-)-Methyltransferases/genetics*
  • DNA (Cytosine-5-)-Methyltransferases/metabolism
  • DNA (Cytosine-5-)-Methyltransferases/physiology
  • DNA Methylation*
  • Evolution, Molecular
  • Fetal Proteins
  • Gene Expression Regulation, Developmental/physiology*
  • Oligonucleotides
  • T-Box Domain Proteins/genetics*
  • T-Box Domain Proteins/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
  • Zebrafish Proteins/physiology
PubMed: 15937923 Full text @ Dev. Dyn.
The zebrafish no tail gene (ntl) is indispensable for tail and notochord development. We have shown previously that ntl is de novo methylated during early embryogenesis. To find the gene that de novo methylates ntl and understand the meaning of this methylation, we cloned seven genes that encode the conserved catalytic domain of methyltransferases. We found that injection of antisense morpholino oligonucleotides against one of them, termed dnmt7, into eggs significantly reduced the level of ntl methylation, although no apparent phenotype was induced by the injection. Inhibition of Dnmt7 activity did not change the level of genome-wide methylation nor did it affect de novo methylation of injected plasmid DNA, indicating that Dnmt7 specifically methylates ntl in the genome. Developmental Dynamics, 2005. (c) 2005 Wiley-Liss, Inc.