PUBLICATION

Staggered cell-intrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina

Authors
Kay, J.N., Link, B.A., and Baier, H.
ID
ZDB-PUB-050506-5
Date
2005
Source
Development (Cambridge, England)   132(11): 2573-2585 (Journal)
Registered Authors
Baier, Herwig, Kay, Jeremy, Link, Brian
Keywords
Zebrafish, ath5 (atoh7), Proneural genes, Atonal, Sonic Hedgehog
MeSH Terms
  • Animals
  • Cell Differentiation/physiology*
  • Chimera/physiology
  • DNA Primers
  • DNA-Binding Proteins/metabolism*
  • Ganglia, Sensory/embryology*
  • Gene Expression Regulation, Developmental*
  • Growth Substances/metabolism*
  • Hedgehog Proteins
  • Lasers
  • Micromanipulation
  • Retinal Ganglion Cells/physiology*
  • Retinal Ganglion Cells/transplantation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction/genetics
  • Stem Cells/physiology
  • Time Factors
  • Trans-Activators/metabolism
  • Veratrum Alkaloids
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism*
PubMed
15857917 Full text @ Development
Abstract
In the developing nervous system, progenitor cells must decide when to withdraw from the cell cycle and commence differentiation. There is considerable debate whether cell-extrinsic or cell-intrinsic factors are most important for triggering this switch. In the vertebrate retina, initiation of neurogenesis has recently been explained by a 'sequential-induction' model - signals from newly differentiated neurons are thought to trigger neurogenesis in adjacent progenitors, creating a wave of neurogenesis that spreads across the retina in a stereotypical manner. We show here, however, that the wave of neurogenesis in the zebrafish retina can emerge through the independent action of progenitor cells - progenitors in different parts of the retina appear pre-specified to initiate neurogenesis at different times. We provide evidence that midline Sonic hedgehog signals, acting before the onset of neurogenesis, are part of the mechanism that sets the neurogenic timer in these cells. Our results highlight the importance of intrinsic factors for triggering neurogenesis, but they also suggest that early signals can modulate these intrinsic factors to influence the timing of neurogenesis many cell cycles later, thereby potentially coordinating axial patterning with control of neuron number and cell fate.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes