PUBLICATION

Staggered cell-intrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina

Authors
Kay, J.N., Link, B.A., and Baier, H.
ID
ZDB-PUB-050506-5
Date
2005
Source
Development (Cambridge, England)   132(11): 2573-2585 (Journal)
Registered Authors
Baier, Herwig, Kay, Jeremy, Link, Brian
Keywords
Zebrafish, ath5 (atoh7), Proneural genes, Atonal, Sonic Hedgehog
MeSH Terms
  • Animals
  • Reverse Transcriptase Polymerase Chain Reaction
  • Micromanipulation
  • Signal Transduction/genetics
  • Retinal Ganglion Cells/physiology*
  • Retinal Ganglion Cells/transplantation
  • Stem Cells/physiology
  • Gene Expression Regulation, Developmental*
  • DNA-Binding Proteins/metabolism*
  • Ganglia, Sensory/embryology*
  • Lasers
  • Growth Substances/metabolism*
  • Time Factors
  • Chimera/physiology
  • Hedgehog Proteins
  • Zebrafish/embryology*
  • Trans-Activators/metabolism
  • Veratrum Alkaloids
  • Cell Differentiation/physiology*
  • DNA Primers
  • Zebrafish Proteins/metabolism*
(all 21)
PubMed
15857917 Full text @ Development
Abstract
In the developing nervous system, progenitor cells must decide when to withdraw from the cell cycle and commence differentiation. There is considerable debate whether cell-extrinsic or cell-intrinsic factors are most important for triggering this switch. In the vertebrate retina, initiation of neurogenesis has recently been explained by a 'sequential-induction' model - signals from newly differentiated neurons are thought to trigger neurogenesis in adjacent progenitors, creating a wave of neurogenesis that spreads across the retina in a stereotypical manner. We show here, however, that the wave of neurogenesis in the zebrafish retina can emerge through the independent action of progenitor cells - progenitors in different parts of the retina appear pre-specified to initiate neurogenesis at different times. We provide evidence that midline Sonic hedgehog signals, acting before the onset of neurogenesis, are part of the mechanism that sets the neurogenic timer in these cells. Our results highlight the importance of intrinsic factors for triggering neurogenesis, but they also suggest that early signals can modulate these intrinsic factors to influence the timing of neurogenesis many cell cycles later, thereby potentially coordinating axial patterning with control of neuron number and cell fate.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca66TgTransgenic Insertion
    rw021TgTransgenic Insertion
      s220tTgTransgenic Insertion
        s273tTgTransgenic Insertion
          t4
            		Deficiency
            th241
              		Point Mutation
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              No data available
              Fish
              Fish
              atoh7th241/th241
              Df(Chr07)t4/t4
              kca66Tg
              rw021Tg
              s220tTg
              s273tTg
              TL
              1 - 7 of 7
              Show
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              GFPEFGGFP
              1 - 1 of 1
              Show
              Mapping
              No data available
              Your Input Welcome
              We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
              Citation
              Kay, J.N., Link, B.A., and Baier, H. (2005) Staggered cell-intrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina. Development (Cambridge, England). 132(11):2573-2585.