PUBLICATION

Integrinalpha5-Dependent Fibronectin Accumulation for Maintenance of Somite Boundaries in Zebrafish Embryos

Authors
Koshida, S., Kishimoto, Y., Ustumi, H., Shimizu, T., Furutani-Seiki, M., Kondoh, H., and Takada, S.
ID
ZDB-PUB-050413-4
Date
2005
Source
Developmental Cell   8(4): 587-598 (Journal)
Registered Authors
Furutani-Seiki, Makoto, Kishimoto, Yasuyuki, Kondoh, Hisato, Koshida, Sumito, Takada, Shinji
Keywords
none
MeSH Terms
  • Cell Polarity
  • Somites/cytology*
  • Somites/physiology*
  • Body Patterning*
  • Integrin alpha5/genetics
  • Integrin alpha5/metabolism*
  • Morphogenesis
  • In Situ Hybridization
  • Animals
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Epithelium/embryology
  • Gene Expression Regulation, Developmental
  • Fibronectins/genetics
  • Fibronectins/metabolism*
  • Ephrin-B2/genetics
  • Ephrin-B2/metabolism
(all 21)
PubMed
15809040 Full text @ Dev. Cell
Abstract
Boundary formation and epithelialization are crucial processes in the morphological segmentation of vertebrate somites. By a genetic screening procedure with zebrafish, we identified two genes, integrinalpha5 (itga5) and fibronectin (fn), required for these processes. Fibronectin proteins accumulate at somite boundaries in accordance with epithelialization of the somites. Both Fibronectin accumulation and the epithelialization are dependent on itga5, which is expressed in the most medial part of somites. Although somite boundaries are initially formed, but not maintained, in the anterior trunk of the mutant embryos deficient in either gene, their maintenance is defective at all axial levels of embryos deficient for both of these genes. Therefore, Integrinalpha5-directed assembly of Fibronectin appears critical for epithelialization and boundary maintenance of somites. Furthermore, with an additional deficiency in ephrin-B2a, the segmental defect in itga5 or fn mutant embryos is expanded posteriorly, indicating that both Integrin-Fibronectin and Eph-Ephrin systems function cooperatively in maintaining somite boundaries.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kt259
    Point Mutation
    kt293
      Small Deletion
      kt443
        Unknown
        kt451
          Point Mutation
          kt664
            Unknown
            ti1
              Small Deletion
              tl43c
                Point Mutation
                tr233
                  Point Mutation
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Fish
                  Antibodies
                  Orthology
                  Engineered Foreign Genes
                  No data available
                  Mapping