PUBLICATION

The basic helix-loop-helix olig3 establishes the neural plate boundary of the trunk and is necessary for development of the dorsal spinal cord

Authors
Filippi, A., Tiso, N., Deflorian, G., Zecchin, E., Bortolussi, M., and Argenton, F.
ID
ZDB-PUB-050301-3
Date
2005
Source
Proceedings of the National Academy of Sciences of the United States of America   102(12): 4377-4382 (Journal)
Registered Authors
Argenton, Francesco, Bortolussi, Marino, Deflorian, Gianluca, Filippi, Alida, Tiso, Natascia, Zecchin, Elisabetta
Keywords
interneurons; neural crest; olig3; zebrafish; glia
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/physiology*
  • Helix-Loop-Helix Motifs/genetics
  • Helix-Loop-Helix Motifs/physiology
  • Neural Crest/embryology*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Gastrula/cytology
  • Base Sequence
  • Molecular Sequence Data
  • Spinal Cord/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • DNA, Complementary/genetics
  • Transcription Factors/genetics*
  • Transcription Factors/physiology*
  • Oligodeoxyribonucleotides, Antisense/genetics
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • In Situ Hybridization
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
  • Animals
(all 23)
PubMed
15731351 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
olig genes encode a previously unrecognized group of vertebrate-specific basic helix-loop-helix transcription factors. As shown in mice, chickens, and zebrafish, two members of this group, olig1 and olig2, are involved in the differentiation of motoneurons and oligodendrocytes, but nothing is known about the role of the third member, olig3. Here, we show that olig3 plays an essential role in the establishment of the neural crest-lateral neural plate boundary. In zebrafish embryos, morpholino-induced olig3 inactivation dramatically increases the number of neural crest cells, but lateral neural plate fates (interneurons and astrocytes) are missing. Zebrafish swirl mutants that have impaired bone morphogenetic protein signaling and lack neural crest cells display an expanded olig3 expression domain. Moreover, olig3 is up-regulated in mindbomb mutants lacking the neural crest because of an impaired notch signaling, and olig3 repression in such mutants rescues the neural crest. In addition, olig3 regulates ngn1 and deltaA expression in interneuron precursors. Our results indicate that olig3 has an essential proneural activity in the dorsal spinal cord and cooperates with the Delta/Notch regulatory loop to establish the boundary between the neural crest and the lateral neural plate. Thus, a proper regulation of the olig gene family is essential for the formation of three cell types (oligodendrocytes, astrocytes, and neural crest) that are unique to vertebrates.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    sb1TgTransgenic Insertion
      ta52b
        		Point Mutation
        ta72a
          		Point Mutation
          tt250
            		Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            olig2MO2-olig2MRPHLNO
            olig4MO1-olig4MRPHLNO
            olig4MO2-olig4MRPHLNO
            1 - 3 of 3
            Show
            Fish
            Fish
            bmp2bta72a/ta72a (TU)
            chrdtt250/tt250
            mib1ta52b/ta52b
            sb1Tg
            smob641/b641 (AB)
            WT
            WT + MO2-olig4
            1 - 7 of 7
            Show
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            No data available
            Mapping
            No data available
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            Citation
            Filippi, A., Tiso, N., Deflorian, G., Zecchin, E., Bortolussi, M., and Argenton, F. (2005) The basic helix-loop-helix olig3 establishes the neural plate boundary of the trunk and is necessary for development of the dorsal spinal cord. Proceedings of the National Academy of Sciences of the United States of America. 102(12):4377-4382.