ZFIN ID: ZDB-PUB-050104-7
Contrast sensitivity, spatial and temporal tuning of the larval zebrafish optokinetic response
Rinner, O., Rick, J.M., and Neuhauss, S.C.
Date: 2005
Source: Investigative ophthalmology & visual science   46(1): 137-142 (Journal)
Registered Authors: Neuhauss, Stephan, Rick, Jens, Rinner, Oliver
Keywords: none
MeSH Terms:
  • Animals
  • Behavior, Animal/physiology
  • Contrast Sensitivity/physiology*
  • Larva/physiology
  • Light
  • Nystagmus, Optokinetic/physiology*
  • Space Perception/physiology*
  • Zebrafish/physiology*
PubMed: 15623766 Full text @ Invest. Ophthalmol. Vis. Sci.
purpose. To characterize the quantitative properties of the optokinetic response (OKR) in zebrafish larvae as a tool to test visual performance in genetically modified larvae. methods. Horizontal OKR was triggered in 5-day-old zebrafish larvae by stimulation with projected computer-generated gratings of varying contrast, angular velocity, temporal and spatial frequency, and brightness. Eye movements were analyzed by a custom-made eye tracker based on image analysis. results. The gain of the OKR slow phase was dependent on angular velocity, spatial frequency, and contrast of a moving grating, but largely independent on brightness. Eye velocity was a logarithmically linear function of grating contrast with a slope of approximately 0.8 per log unit contrast. conclusions. The OKR of the larval zebrafish is not scaled for stimulus contrast and spatial frequency. These properties make the OKR a valuable tool to quantify behavioral visual performance such as visual acuity, contrast sensitivity, and light adaptation. This behavioral paradigm will be useful for analyzing visual performance in mutant and gene-knockdown larval zebrafish.