PUBLICATION

Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm

Authors
Hogan, B.M., Hunter, M.P., Oates, A.C., Crowhurst, M.O., Hall, N.E., Heath, J.K., Prince, V.E., and Lieschke, G.J.
ID
ZDB-PUB-041208-9
Date
2004
Source
Developmental Biology   276(2): 508-522 (Journal)
Registered Authors
Crowhurst, Meredith, Heath, Joan K., Hogan, Ben M., Hunter, Michael, Lieschke, Graham J., Oates, Andrew, Prince, Victoria E.
Keywords
none
MeSH Terms
  • Neuropeptides/classification
  • Neuropeptides/genetics
  • Neuropeptides/metabolism*
  • Sequence Alignment
  • Animals
  • Gene Expression Regulation, Developmental
  • Ectoderm/physiology*
  • Transcription Factors
  • Humans
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • DNA-Binding Proteins
  • Base Sequence
  • Epithelium/metabolism
  • Morphogenesis
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Phylogeny
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Pharynx/embryology*
  • Mice
  • Amino Acid Sequence
  • In Situ Hybridization
  • Molecular Sequence Data
  • Gills/embryology*
  • Parathyroid Glands/embryology
  • Trans-Activators/classification
  • Trans-Activators/genetics
  • Trans-Activators/metabolism*
(all 33)
PubMed
15581882 Full text @ Dev. Biol.
Abstract
The pharyngeal arches give rise to multiple organs critical for diverse processes, including the thymus, thyroid and parathyroids. Several molecular regulators of thymus and thyroid organogenesis are strikingly conserved between mammals and zebrafish. However, land animals have parathyroids whereas fish have gills. The murine transcription factor Glial cells missing 2 (Gcm2) is expressed specifically in the parathyroid primordium in the endodermal epithelium of the third pharyngeal pouch, and in both mice and humans is required for normal development of parathyroid glands. The molecular regulation of fish gill organogenesis remains to be described. We report the expression of gcm2 in the zebrafish pharyngeal epithelium and a requirement for Hox group 3 paralogs for gcm2 expression. Strikingly, zebrafish gcm2 is expressed in the ectodermal portion of the pharyngeal epithelium and is required for the development of the gill filament buds, precursors of fish-specific gill filaments. This study identifies yet another role for a GCM gene in embryonic development and indicates a role for gcm2 during the evolution of divergent pharyngeal morphologies.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
foxi1_unspecified
    		Unspecified
    sox32_unspecified
      		Unspecified
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      gcm2MO1-gcm2MRPHLNO
      gcm2MO2-gcm2MRPHLNO
      gcm2MO3-gcm2MRPHLNO
      hoxa3aMO1-hoxa3aMRPHLNO
      hoxa3aMO2-hoxa3aMRPHLNO
      hoxb3aMO2-hoxb3aMRPHLNO
      hoxb3aMO1-hoxb3aMRPHLNO
      1 - 7 of 7
      Show
      Fish
      Antibodies
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Hogan, B.M., Hunter, M.P., Oates, A.C., Crowhurst, M.O., Hall, N.E., Heath, J.K., Prince, V.E., and Lieschke, G.J. (2004) Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm. Developmental Biology. 276(2):508-522.