PUBLICATION
Conserved and acquired features of neurogenin1 regulation
- Authors
- Blader, P., Lam, C.S., Rastegar, S., Scardigli, R., Nicod, J.C., Simplicio, N., Plessy, C., Fischer, N., Schuurmans, C., Guillemot, F., and Strähle, U.
- ID
- ZDB-PUB-041022-5
- Date
- 2004
- Source
- Development (Cambridge, England) 131(22): 5627-5637 (Journal)
- Registered Authors
- Blader, Patrick, Fischer, Nadine, Lam, Eric (C.S.), Plessy, Charles, Rastegar, Sepand, Strähle, Uwe
- Keywords
- neurogenin1, Pax6, Telencephalon, Diencephalon, Evolution, Neurogenesis, Transcription, Regulatory elements, Co-option, Zebrafish, Mouse
- MeSH Terms
-
- Conserved Sequence/genetics*
- Protein Binding
- Telencephalon/cytology
- Telencephalon/embryology
- Telencephalon/metabolism
- Animals
- Molecular Sequence Data
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Transgenes/genetics
- Transcription, Genetic/genetics
- Mice
- Basic Helix-Loop-Helix Transcription Factors
- Base Sequence
- Sequence Alignment
- Response Elements/genetics
- Zebrafish Proteins/genetics*
- Transcription Factors/genetics*
- Humans
- Repressor Proteins
- Animals, Genetically Modified
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Organ Specificity
- Paired Box Transcription Factors
- Rhombencephalon/embryology
- Rhombencephalon/metabolism
- Nerve Tissue Proteins/genetics*
- Eye Proteins
- Gene Expression Regulation, Developmental*
- Regulatory Sequences, Nucleic Acid/genetics*
- Enhancer Elements, Genetic/genetics
- Amino Acid Sequence
- PubMed
- 15496438 Full text @ Development
Citation
Blader, P., Lam, C.S., Rastegar, S., Scardigli, R., Nicod, J.C., Simplicio, N., Plessy, C., Fischer, N., Schuurmans, C., Guillemot, F., and Strähle, U. (2004) Conserved and acquired features of neurogenin1 regulation. Development (Cambridge, England). 131(22):5627-5637.
Abstract
The telencephalon shows vast morphological variations among different vertebrate groups. The transcription factor neurogenin1 (ngn1) controls neurogenesis in the mouse pallium and is also expressed in the dorsal telencephalon of the evolutionary distant zebrafish. The upstream regions of the zebrafish and mammalian ngn1 loci harbour several stretches of conserved sequences. Here, we show that the upstream region of zebrafish ngn1 is capable of faithfully recapitulating endogenous expression in the zebrafish and mouse telencephalon. A single conserved regulatory region is essential for dorsal telencephalic expression in the zebrafish, and for expression in the dorsal pallium of the mouse. However, a second conserved region that is inactive in the fish telencephalon is necessary for expression in the lateral pallium of mouse embryos. This regulatory region, which drives expression in the zebrafish diencephalon and hindbrain, is dependent on Pax6 activity and binds recombinant Pax6 in vitro. Thus, the regulatory elements of ngn1 appear to be conserved among vertebrates, with certain differences being incorporated in the utilisation of these enhancers, for the acquisition of more advanced features in amniotes. Our data provide evidence for the co-option of regulatory regions as a mechanism of evolutionary diversification of expression patterns, and suggest that an alteration in Pax6 expression was crucial in neocortex evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping