PUBLICATION
Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains
- Authors
- Kudoh, T., Concha, M.L., Houart, C., Dawid, I.B., and Wilson, S.W.
- ID
- ZDB-PUB-040721-7
- Date
- 2004
- Source
- Development (Cambridge, England) 131(15): 3581-3592 (Journal)
- Registered Authors
- Concha, Miguel, Dawid, Igor B., Houart, Corinne, Kudoh, Tetsuhiro, Wilson, Steve
- Keywords
- Zebrafish, Default model, Neural induction
- MeSH Terms
-
- Gene Expression Regulation, Developmental
- Fibroblast Growth Factors/metabolism*
- Zebrafish/embryology
- Animals
- Signal Transduction/physiology*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Bone Morphogenetic Proteins/antagonists & inhibitors
- Bone Morphogenetic Proteins/metabolism*
- Cell Lineage
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- High Mobility Group Proteins/genetics
- High Mobility Group Proteins/metabolism
- Glycoproteins/genetics
- Glycoproteins/metabolism
- Cell Movement
- Organizers, Embryonic
- Central Nervous System/embryology*
- In Situ Hybridization
- Biomarkers
- Intercellular Signaling Peptides and Proteins/genetics
- Intercellular Signaling Peptides and Proteins/metabolism
- SOXB1 Transcription Factors
- Gastrula*/cytology
- Gastrula*/physiology
- Ectoderm/cytology
- Ectoderm/physiology*
- Transcription Factors
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Body Patterning*
- PubMed
- 15262889 Full text @ Development
Citation
Kudoh, T., Concha, M.L., Houart, C., Dawid, I.B., and Wilson, S.W. (2004) Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains. Development (Cambridge, England). 131(15):3581-3592.
Abstract
Studies in fish and amphibia have shown that graded Bmp signalling activity regulates dorsal-to-ventral (DV) patterning of the gastrula embryo. In the ectoderm, it is thought that high levels of Bmp activity promote epidermal development ventrally, whereas secreted Bmp antagonists emanating from the organiser induce neural tissue dorsally. However, in zebrafish embryos, the domain of cells destined to contribute to the spinal cord extends all the way to the ventral side of the gastrula, a long way from the organiser. We show that in vegetal (trunk and tail) regions of the zebrafish gastrula, neural specification is initiated at all DV positions of the ectoderm in a manner that is unaffected by levels of Bmp activity and independent of organiser-derived signals. Instead, we find that Fgf activity is required to induce vegetal prospective neural markers and can do so without suppressing Bmp activity. We further show that Bmp signalling does occur within the vegetal prospective neural domain and that Bmp activity promotes the adoption of caudal fate by this tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping