PUBLICATION
Visualisation of Zebrafish infection by GFP-labelled Vibrio anguillarum
- Authors
- O'Toole, R., von Hofsten, J., Rosqvist, R., Olsson, P.E., and Wolf-Watz, H.
- ID
- ZDB-PUB-040616-2
- Date
- 2004
- Source
- Microbial pathogenesis 37(1): 41-46 (Journal)
- Registered Authors
- von Hofsten, Jonas
- Keywords
- none
- MeSH Terms
-
- Flagella/physiology
- Zebrafish/microbiology*
- Staining and Labeling/methods*
- Movement
- Genes, Reporter
- Gastrointestinal Tract/microbiology
- Gastrointestinal Tract/pathology
- Vibrio/pathogenicity*
- Vibrio/physiology
- Chemotaxis
- Vibrio Infections/microbiology*
- Vibrio Infections/pathology
- Skin/microbiology
- Skin/pathology
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism*
- Green Fluorescent Proteins
- Animals
- Bacterial Adhesion
- PubMed
- 15194159 Full text @ Microb. Pathog.
Citation
O'Toole, R., von Hofsten, J., Rosqvist, R., Olsson, P.E., and Wolf-Watz, H. (2004) Visualisation of Zebrafish infection by GFP-labelled Vibrio anguillarum. Microbial pathogenesis. 37(1):41-46.
Abstract
Vibrio anguillarum is an invasive pathogen of fish causing a septicaemia called vibriosis. In this work, transparent zebrafish were immersed in water containing green fluorescent protein labelled V. anguillarum. The infection was visualised at the whole fish and single bacterium levels using microscopy. The gastrointestinal tract was the first site where the pathogen was detected. This enteric localisation occurred independently of the flagellum or motility. On the other hand, chemotactic motility was essential for association of the pathogen with the fish surface. In conclusion, the zebrafish infection model provides evidence that the intestine and skin represent sites of infection by V. anguillarum and suggests a host site where chemotaxis may function in virulence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping