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ZFIN ID: ZDB-PUB-031229-3
Neural cell fate analysis in zebrafish using olig2 BAC transgenics
Shin, J., Park, H.-C., Topczewska, J.M., Mawdsley, D.J., and Appel, B.
Date: 2003
Source: Methods in cell science : an official journal of the Society for In Vitro Biology 25(1-2): 7-14 (Review)
Registered Authors: Appel, Bruce, Mawdsley, David, Park, Hae-Chul, Shin, Jimann, Topczewska, Jolanta
Keywords: cerebellum, EGFP, motor neuron, olig2, oligodendrocyte, Purkinje neuron, spinal cord, transgenic, zebrafish
MeSH Terms:
  • Animals
  • Cloning, Molecular
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental/physiology*
  • Genes, Reporter/genetics
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Luminescent Proteins/metabolism
  • Microinjections
  • Motor Neurons/cytology
  • Motor Neurons/metabolism*
  • Peripheral Nervous System/cytology
  • Peripheral Nervous System/metabolism*
  • Purkinje Cells/cytology
  • Purkinje Cells/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 14739582 Full text @ Methods Cell Sci.
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ABSTRACT
We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
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