PUBLICATION

Histology-based screen for zebrafish mutants with abnormal cell differentiation

Authors
Mohideen, M.A., Beckwith, L.G., Tsao-Wu, G.S., Moore, J.L., Wong, A.C., Chinoy, M.R., and Cheng, K.C.
ID
ZDB-PUB-031103-7
Date
2003
Source
Developmental dynamics : an official publication of the American Association of Anatomists   228(3): 414-423 (Journal)
Registered Authors
Beckwith, Lee G., Cheng, Keith C., Mohideen, Manzoor Pallithotangal, Moore, Jessica L., Tsao-Wu, Gladys, Wong, Andrew
Keywords
zebrafish mutant, larval arrays, histology, cell differentiation, retina, cancer
MeSH Terms
  • Animals
  • Cell Differentiation/genetics*
  • Chromosome Mapping
  • Eye/cytology
  • Eye/embryology
  • Male
  • Morphogenesis
  • Mutagenesis*
  • Parthenogenesis
  • Photoreceptor Cells, Vertebrate/cytology
  • Zebrafish/genetics*
PubMed
14579380 Full text @ Dev. Dyn.
Abstract
The power of histology to define states of cell differentiation was used as the basis of a mutagenesis screen in zebrafish. In this screen, 7-day-old parthenogenetic half-tetrad larvae from potential carrier females were screened for mutations affecting cell differentiation in hematoxylin and eosin-stained tissue sections. Seven, noncomplementing, recessive mutations were found. Two mutations affect only the retina: segmented photoreceptors (spr) show a discontinuous photoreceptor cell layer; vestigial outer segments (vos) has fewer photoreceptor cells and degenerated outer segments within this cell layer. Three mutants have gut-specific defects: the epithelial cells of kirby (kby) are replaced by ballooned cells; the intestines of stuffy (sfy) and stuffed (sfd) contain increased luminal mucus. Two mutations affect multiple organs: disordered neural retina (dnr) has disrupted retinal layering and mild nuclear abnormalities in the gut and liver; and in huli hutu (hht), the retinal cell layers are disorganized and multiple organs have mild to severe nuclear abnormalities that are reminiscent of the atypia of human neoplasia. Each mutation appears to be homozygous lethal. This screen is proof of principle for the feasibility of histologic screens to yield novel mutations, including potential models of human disease. The throughput for this type of screen may be enhanced by automation.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping