PUBLICATION

A cellular framework for gut-looping morphogenesis in zebrafish

Authors
Horne-Badovinac, S., Rebagliati, M., and Stainier, D.Y.
ID
ZDB-PUB-031103-19
Date
2003
Source
Science (New York, N.Y.)   302(5645): 662-665 (Journal)
Registered Authors
Horne-Badovinac, Sally, Rebagliati, Michael, Stainier, Didier
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning*
  • Cell Movement
  • Cues
  • Endoderm/physiology
  • Gene Expression Regulation, Developmental*
  • Guanylate Kinases
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Intestines/embryology*
  • Isoenzymes
  • Mesoderm/cytology
  • Mesoderm/physiology
  • Morphogenesis
  • Mutation
  • Nuclear Proteins*
  • Nucleoside-Phosphate Kinase/genetics
  • Nucleoside-Phosphate Kinase/metabolism
  • Oligonucleotides, Antisense
  • Phenotype
  • Protein Kinase C/genetics
  • Protein Kinase C/physiology
  • Transcription Factors/genetics
  • Transcription Factors/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology
PubMed
14576439 Full text @ Science
Abstract
Many vertebrate organs adopt asymmetric positions with respect to the midline, but little is known about the cellular changes and tissue movements that occur downstream of left-right gene expression to produce this asymmetry. Here, we provide evidence that the looping of the zebrafish gut results from the asymmetric migration of the neighboring lateral plate mesoderm (LPM). Mutations that disrupt the epithelial structure of the LPM perturb this asymmetric migration and inhibit gut looping. Asymmetric LPM migration still occurs when the endoderm is ablated from the gut-looping region, suggesting that the LPM can autonomously provide a motive force for gut displacement. Finally, reducing left-sided Nodal activity randomizes the pattern of LPM migration and gut looping. These results reveal a cellular framework for the regulation of organ laterality by asymmetrically expressed genes.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes