PUBLICATION
In vivo regulation of the chicken cardiac troponin T gene promoter in zebrafish embryos
- Authors
- Tidyman, W.E., Sehnert, A.J., Huq, A., Agard, J., Deegan, F., Stainier, D.Y., and Ordahl, C.P.
- ID
- ZDB-PUB-030805-7
- Date
- 2003
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 227(4): 484-496 (Journal)
- Registered Authors
- Huq, Anja, Sehnert, Amy, Stainier, Didier
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Base Sequence
- Binding Sites/genetics
- Cells, Cultured
- Chick Embryo
- Chickens/genetics*
- Enhancer Elements, Genetic/genetics
- Evolution, Molecular*
- Gene Components
- Gene Expression Regulation, Developmental*
- Green Fluorescent Proteins
- In Situ Hybridization
- Luciferases
- Luminescent Proteins
- Microinjections
- Muscles/embryology
- Mutation/genetics
- Plasmids
- Promoter Regions, Genetic/genetics
- Troponin T/genetics*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 12889057 Full text @ Dev. Dyn.
Citation
Tidyman, W.E., Sehnert, A.J., Huq, A., Agard, J., Deegan, F., Stainier, D.Y., and Ordahl, C.P. (2003) In vivo regulation of the chicken cardiac troponin T gene promoter in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 227(4):484-496.
Abstract
The chicken cardiac troponin T (cTnT) gene is representative of numerous cardiac and skeletal muscle-specific genes that contain muscle-CAT (MCAT) elements within their promoters. We examined the regulation of the chicken cTnT gene in vivo in zebrafish embryos, and in vitro in cardiomyocyte, myoblast, and fibroblast cultures. Defined regions of the cTnT promoter were linked to the green fluorescent protein (GFP) gene for in vivo analysis, and the luciferase gene for in vitro analysis. Injection of the cTnT promoter constructs into fertilized zebrafish eggs resulted in GFP expression in both heart and skeletal muscle cells reproducing the pattern of expression of the endogenous cTnT gene in the chicken embryo. Promoter deletion analysis revealed that the cis-regulatory regions responsible for cardiac and skeletal muscle-specific expression functioned in an equivalent manner in both in vitro and in vivo environments. In addition, we show that mutation of the poly-ADP ribose polymerase-I (PARP-I) binding site adjacent to the distal MCAT element in the chicken cTnT promoter produced a non-cell-specific promoter in vitro and in the zebrafish. Thus, the PARP-I transcriptional regulatory mechanism that governs muscle specificity of the chicken cTnT promoter is conserved across several chordate classes spanning at least 350 million years of evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping