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ZFIN ID: ZDB-PUB-030730-8
The ventralized ogon mutant phenotype is caused by a mutation in the zebrafish homologue of Sizzled, a secreted Frizzled-related protein
Martyn, U. and Schulte-Merker, S.
Date: 2003
Source: Developmental Biology   260(1): 58-67 (Journal)
Registered Authors: Martyn, Ulrike, Schulte-Merker, Stefan
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Body Patterning/genetics
  • Bone Morphogenetic Proteins/deficiency
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Embryo, Nonmammalian
  • Evolution, Molecular
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Open Reading Frames
  • Phenotype
  • Phylogeny
  • Point Mutation
  • Protein Structure, Tertiary
  • Proteins/chemistry
  • Proteins/genetics*
  • Proteins/metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 12885555 Full text @ Dev. Biol.
The BMP signaling pathway plays a key role during dorsoventral pattern formation of vertebrate embryos. In zebrafish, all cloned mutants affecting this process are deficient in members of the BMP pathway. In a search for factors differentially expressed in swirl/bmp2b mutants compared with wild type, we isolated zebrafish Sizzled, a member of the secreted Frizzled-related protein family and putative Wnt inhibitor. The knockdown of sizzled using antisense morpholino phenocopied the ventralized mutant ogon (formerly also known as mercedes and short tail). By sequencing and rescue experiments, we demonstrate that ogon encodes sizzled. Overexpression of sizzled, resulting in strongly dorsalized phenotypes, and the expression domains of sizzled in wild type embryos, localized in the ventral side during gastrulation and restricted to the posterior end during segmentation stages, correlate with its role in dorsoventral patterning. The expanded expression domain of sizzled in ogon and chordino together with its downregulation in swirl suggests a BMP2b-dependent negative autoregulation of sizzled. Indicating a novel role for a secreted Frizzled-related protein, we show that, in addition to the BMP pathway, a component of the Wnt signaling pathway is required for dorsoventral pattern formation in zebrafish.