PUBLICATION

The ventralized ogon mutant phenotype is caused by a mutation in the zebrafish homologue of Sizzled, a secreted Frizzled-related protein

Authors
Martyn, U. and Schulte-Merker, S.
ID
ZDB-PUB-030730-8
Date
2003
Source
Developmental Biology   260(1): 58-67 (Journal)
Registered Authors
Martyn, Ulrike, Schulte-Merker, Stefan
Keywords
none
MeSH Terms
  • Evolution, Molecular
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Bone Morphogenetic Proteins/deficiency
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Body Patterning/genetics
  • Animals
  • Gene Expression Regulation, Developmental
  • Phenotype
  • Open Reading Frames
  • Amino Acid Sequence
  • Protein Structure, Tertiary
  • Point Mutation
  • Embryo, Nonmammalian
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Proteins/chemistry
  • Proteins/genetics*
  • Proteins/metabolism
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Base Sequence
  • Signal Transduction
(all 26)
PubMed
12885555 Full text @ Dev. Biol.
Abstract
The BMP signaling pathway plays a key role during dorsoventral pattern formation of vertebrate embryos. In zebrafish, all cloned mutants affecting this process are deficient in members of the BMP pathway. In a search for factors differentially expressed in swirl/bmp2b mutants compared with wild type, we isolated zebrafish Sizzled, a member of the secreted Frizzled-related protein family and putative Wnt inhibitor. The knockdown of sizzled using antisense morpholino phenocopied the ventralized mutant ogon (formerly also known as mercedes and short tail). By sequencing and rescue experiments, we demonstrate that ogon encodes sizzled. Overexpression of sizzled, resulting in strongly dorsalized phenotypes, and the expression domains of sizzled in wild type embryos, localized in the ventral side during gastrulation and restricted to the posterior end during segmentation stages, correlate with its role in dorsoventral patterning. The expanded expression domain of sizzled in ogon and chordino together with its downregulation in swirl suggests a BMP2b-dependent negative autoregulation of sizzled. Indicating a novel role for a secreted Frizzled-related protein, we show that, in addition to the BMP pathway, a component of the Wnt signaling pathway is required for dorsoventral pattern formation in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Martyn, U. and Schulte-Merker, S. (2003) The ventralized ogon mutant phenotype is caused by a mutation in the zebrafish homologue of Sizzled, a secreted Frizzled-related protein. Developmental Biology. 260(1):58-67.