PUBLICATION
Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos
- Authors
- Raya, A., Kawakami, Y., Rodriguez-Esteban, C., Büscher, D., Koth, C.M., Itoh, T., Morita, M., Raya, R.M., Dubova, I., Bessa, J.G., de la Pompa, J.L., and Izpisúa Belmonte, J.C.
- ID
- ZDB-PUB-030527-25
- Date
- 2003
- Source
- Genes & Development 17(10): 1213-1218 (Journal)
- Registered Authors
- Büscher, Dirk, Dubova, Ilir, M.D., Itoh, Tohru, Izpisúa Belmonte, Juan Carlos, Kawakami, Yasuhiko, Koth, Chris, Raya, Angel, Raya, Marina, Rodriguez-Esteban, Concepcion
- Keywords
- none
- MeSH Terms
-
- Transforming Growth Factor beta/genetics*
- Transforming Growth Factor beta/metabolism
- Gene Expression Regulation/physiology*
- Nodal Protein
- Mice
- Situs Inversus/embryology
- Body Patterning/physiology*
- Trans-Activators/genetics
- Trans-Activators/metabolism
- Hedgehog Proteins
- Enhancer Elements, Genetic
- Zebrafish/embryology
- Zebrafish/genetics
- Signal Transduction/physiology
- Membrane Proteins/physiology*
- Animals
- Receptors, Notch
- Organizers, Embryonic/physiology
- Promoter Regions, Genetic
- PubMed
- 12730123 Full text @ Genes & Dev.
Citation
Raya, A., Kawakami, Y., Rodriguez-Esteban, C., Büscher, D., Koth, C.M., Itoh, T., Morita, M., Raya, R.M., Dubova, I., Bessa, J.G., de la Pompa, J.L., and Izpisúa Belmonte, J.C. (2003) Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos. Genes & Development. 17(10):1213-1218.
Abstract
Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping