PUBLICATION

Myc-induced T cell leukemia in transgenic zebrafish

Authors
Langenau, D.M., Traver, D., Ferrando, A.A., Kutok, J.L., Aster, J.C., Kanki, J.P., Lin, S., Prochownik, E., Trede, N.S., Zon, L.I., and Look, A.T.
ID
ZDB-PUB-030211-10
Date
2003
Source
Science (New York, N.Y.)   299(5608): 887-890 (Journal)
Registered Authors
Kanki, John, Langenau, David, Lin, Shuo, Look, A. Thomas, Traver, David, Trede, Nick, Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Lineage
  • Cell Transformation, Neoplastic*
  • Clone Cells
  • DNA-Binding Proteins/genetics
  • Disease Models, Animal*
  • Female
  • Fertilization in Vitro
  • Gene Expression Profiling
  • Genes, myc*
  • Green Fluorescent Proteins
  • Kidney/pathology
  • Leukemia-Lymphoma, Adult T-Cell*/genetics
  • Leukemia-Lymphoma, Adult T-Cell*/pathology
  • Leukemic Infiltration
  • Luminescent Proteins/metabolism
  • Male
  • Mice
  • Mutation
  • Neoplasm Transplantation
  • Olfactory Bulb/pathology
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins/metabolism
  • Spleen/pathology
  • T-Lymphocytes/immunology
  • T-Lymphocytes/pathology*
  • T-Lymphocytes/physiology
  • Thymus Gland/pathology
  • Transgenes
  • Zebrafish*/embryology
  • Zebrafish*/genetics
PubMed
12574629 Full text @ Science
Abstract
The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-myc under control of the zebrafish Rag2 promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping