ZFIN ID: ZDB-PUB-021202-1
Production of maternal-zygotic mutant zebrafish by germ-line replacement
Ciruna, B., Weidinger, G., Knaut, H., Thisse, B., Thisse, C., Raz, E., and Schier, A.F.
Date: 2002
Source: Proc. Natl. Acad. Sci. USA 99(23): 14919-14924 (Journal)
Registered Authors: Ciruna, Brian, Knaut, Holger, Raz, Erez, Schier, Alexander, Thisse, Bernard, Thisse, Christine, Weidinger, Gilbert
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Cell Transplantation
  • DNA Primers
  • Female
  • Genomic Imprinting*
  • Genotype
  • Germ-Line Mutation
  • Male
  • RNA, Messenger/genetics
  • Transplantation Chimera
  • Zebrafish/genetics*
  • Zygote/physiology*
PubMed: 12397179 Full text @ Proc. Natl. Acad. Sci. USA
FIGURES
ABSTRACT
We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of germ cells derived from homozygous mutant donors. Successful transfers are identified by the localization of specifically labeled donor PGCs to the region of the developing gonad in chimeric embryos. This strategy, which results in the complete replacement of the host germ line with donor PGCs, was validated by the generation of maternal and maternal-zygotic mutants for the miles apart locus. This germ-line replacement technique provides a powerful tool for studying the maternal effects of zygotic lethal mutations. Furthermore, the ability to generate large clutches of purely mutant embryos will greatly facilitate embryological, genetic, genomic, and biochemical studies.
ADDITIONAL INFORMATIONNo data available