Up-regulation of cadherin-2 and cadherin-4 in regenerating visual structures of adult zebrafish
- Liu, Q., Londraville, R.L., Azodi, E., Babb, S.G., Chiappini-Williamson, C., Marrs, J.A., and Raymond, P.A.
- Experimental neurology 177(2): 396-406 (Journal)
- Registered Authors
- Clendenon, Sherry, Liu, Qin, Marrs, James A., Raymond, Pamela
- zebrafish; regeneration; retina; optic pathway; cell adhesion molecules
- MeSH Terms
- Eye Injuries, Penetrating/pathology
- Eye Injuries, Penetrating/physiopathology
- Models, Animal
- Nerve Crush
- Nerve Regeneration/physiology*
- Optic Nerve/cytology
- Optic Nerve/physiology
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/physiology
- Superior Colliculi/cytology
- Superior Colliculi/metabolism
- Time Factors
- Visual Pathways/cytology
- Visual Pathways/metabolism*
- Wound Healing/physiology
- Zebrafish Proteins/metabolism
- 12429186 Full text @ Exp. Neurol.
Liu, Q., Londraville, R.L., Azodi, E., Babb, S.G., Chiappini-Williamson, C., Marrs, J.A., and Raymond, P.A. (2002) Up-regulation of cadherin-2 and cadherin-4 in regenerating visual structures of adult zebrafish. Experimental neurology. 177(2):396-406.
Cadherins are homophilic cell adhesion molecules that control development of a variety of tissues and maintenance of adult structures In this study, we examined expression of zebrafish cadherin-2 (Cdh2, N -cadherin) and cadherin-4 (Cdh4, R-cadherin) in the visual system of adult zebrafish after eye or optic nerve lesions using immunocytochemistry and immunoblotting. Both Cdh2 and Cdh4 immunoreactivities were specifically up-regulated in regenerating retina and/or the optic pathway. Furthermore, temporal expression patterns of these two cadherins were distinct during the regeneration of the injured tissues. Cadherins have been shown to regulate axonal outgrowth in the developing nervous system, but this is the first report, to our knowledge, of increased cadherin expression associated with axonal regeneration in the vertebrate central nervous system. Our results suggest that both Cdh2 and Cdh4 may be important for regeneration of injured retinal ganglion cell axons.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes