PUBLICATION
cDNA cloning and expressions of cytochrome P450 1A in zebrafish embryos
- Authors
- Yamazaki, K., Teraoka, H., Dong, W., Stegeman, J.J., and Hiraga, T.
- ID
- ZDB-PUB-021105-13
- Date
- 2002
- Source
- The Journal of veterinary medical science 64(9): 829-833 (Journal)
- Registered Authors
- Dong, Wu, Stegeman, John J., Teraoka, Hiroki
- Keywords
- none
- MeSH Terms
-
- Molecular Sequence Data
- Cytochrome P-450 CYP1A1/analysis*
- Cytochrome P-450 CYP1A1/biosynthesis
- Cytochrome P-450 CYP1A1/genetics*
- Amino Acid Sequence
- PubMed
- 12399609 Full text @ J. Vet. Med. Sci.
Abstract
Cytochrome P450 1A (CYP1A) is well known for being induced by aromatic hydrocarbons, including 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). We determined the complete cDNA sequence of a CYP1A open reading frame with both 5'- and 3'-ends in zebrafish (zfCYP1A), a useful model for environmental toxicology. zfCYP1A shows high percentage identity with CYP1As of mammals, domestic fowl and xenopus (51.9-60.4%), as well as the other fish species (63.8-89.2%). As revealed by in situ hybridization and immunohistochemistry, zfCYP1A was scarcely detected in control embryos but was markedly induced by TCDD especially in heart, vascular endothelial cells, intestinal epithelium, pronephros and outer integument in both prehatched and hatched embryos. These expression patterns are consistent with possible involvement of zfCYP1A in TCDD- induced toxicities.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping