PUBLICATION

The homeobox gene mbx is involved in eye and tectum development

Authors
Kawahara, A., Chien, C.-B., and Dawid, I.B.
ID
ZDB-PUB-020807-10
Date
2002
Source
Developmental Biology   248(1): 107-117 (Journal)
Registered Authors
Chien, Chi-Bin, Dawid, Igor B., Kawahara, Atsuo
Keywords
none
MeSH Terms
  • Recombinant Fusion Proteins/metabolism
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Eye/embryology*
  • Phylogeny
  • RNA, Messenger/metabolism
  • Brain/embryology
  • Zebrafish
  • Animals
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • In Situ Nick-End Labeling
  • Amino Acid Sequence
  • Time Factors
  • Gene Expression Regulation
  • Phenotype
  • Paired Box Transcription Factors
  • Mitosis
  • Repressor Proteins
  • Embryo, Nonmammalian/metabolism
  • Gastrula/metabolism
  • Superior Colliculi/embryology*
  • Apoptosis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Oligonucleotides/pharmacology
  • Protein Structure, Tertiary
  • Eye Proteins
  • Humans
  • Sequence Homology, Amino Acid
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • In Situ Hybridization
(all 34)
PubMed
12142024 Full text @ Dev. Biol.
Abstract
The homeobox gene mbx is first activated at the end of gastrulation in zebrafish in the presumptive forebrain and midbrain region. During somitogenesis stages, the anterior expression of mbx, which partly overlaps the future eye field, gradually decreases, while midbrain expression intensifies and becomes restricted to the presumptive tectum . Knockdown of mbx expression by morpholino antisense oligonucleotides ( mbx-MO) leads to a reduction in the size of the eyes and tectum. Expression domains of rx1 and pax6 in the eye field and of mab21l2 in the eye field and tectum anlage were reduced in size in mbx-MO-injected embryos by somitogenesis stages. Further, induction of islet1 and lim3 expression in the eye at 2 days postfertilization (dpf) was suppressed in mbx-MO-injected embryos. In mbx-MO-injected embryos at 2-5 dpf, the lamination of the eye was disorganized and the number of retinal axons was substantially reduced, but the few remaining axons navigated appropriately to the contralateral tectum. A chimeric protein composed of the Mbx DNA-binding domain and the VP16 activation domain affected eye and tectum development similarly to mbx-MO knockdown, suggesting that Mbx acts as a transcriptional repressor in the zebrafish embryo. Based on these data, we propose that the mbx homeobox gene is required for the development of the eyes and tectum.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tc300a
    		Point Mutation
    ti282a
      		Point Mutation
      tt250
        		Point Mutation
        tu29a
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          dmbx1aMO1-dmbx1aMRPHLNO
          dmbx1aMO2-dmbx1aMRPHLNO
          1 - 2 of 2
          Show
          Fish
          Fish
          bmp2btc300a/tc300a (TU)
          chrdtt250/tt250
          fgf8ati282a/ti282a
          pax2atu29a/tu29a
          1 - 4 of 4
          Show
          Antibodies
          No data available
          Orthology
          Gene Orthology
          dmbx1a
          1 - 1 of 1
          Show
          Engineered Foreign Genes
          No data available
          Mapping
          No data available
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          Citation
          Kawahara, A., Chien, C.-B., and Dawid, I.B. (2002) The homeobox gene mbx is involved in eye and tectum development. Developmental Biology. 248(1):107-117.