PUBLICATION
Zebrafish insulin-like growth factor-I receptor: molecular cloning and developmental expression
- Authors
- Ayaso, E., Nolan, C.M., and Byrnes, L.
- ID
- ZDB-PUB-020624-5
- Date
- 2002
- Source
- Molecular and Cellular Endocrinology 191(2): 137-148 (Journal)
- Registered Authors
- Ayaso, Elena, Byrnes, Lucy
- Keywords
- IGF; IGF-I receptor; zebrafish; mRNA expression; embryonic development
- MeSH Terms
-
- 3' Untranslated Regions
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary/isolation & purification
- Embryonic and Fetal Development/genetics*
- Gene Expression Regulation, Developmental*
- Genetic Linkage
- Molecular Sequence Data
- Open Reading Frames
- Protein-Tyrosine Kinases/genetics
- Receptor, IGF Type 1/biosynthesis
- Receptor, IGF Type 1/genetics*
- Sequence Homology, Nucleic Acid
- Zebrafish
- PubMed
- 12062897 Full text @ Mol. Cell. Endocrinol.
Citation
Ayaso, E., Nolan, C.M., and Byrnes, L. (2002) Zebrafish insulin-like growth factor-I receptor: molecular cloning and developmental expression. Molecular and Cellular Endocrinology. 191(2):137-148.
Abstract
The biological actions of the insulin-like growth factors (IGFs) are mediated primarily by the IGF-I receptor (IGF-IR), and the IGF family has been highly conserved throughout vertebrate evolution. In this study we report the isolation of a 3 kb cDNA clone for the zebrafish IGF-IR that includes the complete 3' untranslated region and polyA tail and mapping of the receptor gene to zebrafish linkage group 7. The open reading frame deduced from the cDNA sequence encompasses the juxtamembrane and protein tyrosine kinase portions of the receptor, and is 70 and 67% identical to the corresponding regions of the IGF-IRs of the turbot and Xenopus, respectively. By RT-PCR, zebrafish IGF-IR expression was detected from early blastula to early larval stages of development. Using whole mount in situ hybridization, IGF-IR expression was detected after gastrulation. Expression was evident in most tissues but was particularly evident in the tail, in eye and ear primordia and in the brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping