PUBLICATION

Lateral line, nervous system, and maternal expression of Frizzled 7a during zebrafish embryogenesis

Authors
Sumanas, S., Kim, H.J., Hermanson, S.B., and Ekker, S.C.
ID
ZDB-PUB-020618-5
Date
2002
Source
Mechanisms of Development   115(1-2): 107-111 (Journal)
Registered Authors
Ekker, Stephen C., Hermanson, Spencer, Kim, Hyon, Sumanas, Saulius
Keywords
Wnt; frizzled; zebrafish; expression; maternal; nervous system; lateral line; neuromast
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary
  • Expressed Sequence Tags
  • Female
  • Gene Expression*
  • Gene Expression Profiling
  • Mice
  • Molecular Sequence Data
  • Nervous System/embryology
  • Nervous System/metabolism
  • Receptors, Cell Surface/genetics*
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
12049772 Full text @ Mech. Dev.
Abstract
We have isolated and mapped a new wnt receptor frizzled family member, zebrafish frizzled 7a. Fz7a and a previously reported zebrafish fz7 () make an orthologous gene pair, however, they display distinct expression patterns. Fz7a shows strong maternal as well as zygotic expression. Fz7a transcript is enriched dorsally starting with the shield stage. At the end of gastrulation, Fz7a is abundantly expressed within anterior neuroectoderm and expressed more weakly within lateral mesoderm. Fz7a is detected during somitogenesis within the central nervous system, somatic and posterior lateral mesoderm. At 24hpf, fz7a is expressed in migrating lateral line primordium. At 48hpf, fz7a is detected in the ear , pectoral fin bud, and within neuromasts, which had originated from the lateral line primordium. Radiation hybrid mapping using panel LN54 () places fz7a on linkage group 6, linked to the marker fi11h08 (distance 0 .00cR, LOD score 14.1). To prove that fz7 and fz7a are indeed different genes we mapped fz7 as well using the same LN54 panel. Fz7 mapped to linkage group 9 with a LOD of 12.5, 27.31 cR from wnt 10a in between markers IBD2759 and fb50e04.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes