|ZFIN ID: ZDB-PUB-020605-1|
Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development
Brabazon, E.D., Bree, R.T., Carton, M.W., Grealy, M., and Byrnes, L.
|Source:||BBA Gene Structure and Expression 1576(1-2): 203-208 (Journal)|
|Registered Authors:||Brabazon, Elaine, Bree, Ronan, Byrnes, Lucy|
|Keywords:||cyclin-dependent kinase 8; cDNA; expression; zebrafish; embryogenesis; mid-blastula transition|
|PubMed:||12031503 Full text @ BBA Gene Structure and Expression|
Brabazon, E.D., Bree, R.T., Carton, M.W., Grealy, M., and Byrnes, L. (2002) Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development. BBA Gene Structure and Expression. 1576(1-2):203-208.
ABSTRACTCyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid -blastula transition (MBT) and throughout embryonic development.