ZFIN ID: ZDB-PUB-020605-1
Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development
Brabazon, E.D., Bree, R.T., Carton, M.W., Grealy, M., and Byrnes, L.
Date: 2002
Source: BBA Gene Structure and Expression   1576(1-2): 203-208 (Journal)
Registered Authors: Brabazon, Elaine, Bree, Ronan, Byrnes, Lucy
Keywords: cyclin-dependent kinase 8; cDNA; expression; zebrafish; embryogenesis; mid-blastula transition
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 8
  • Cyclin-Dependent Kinases*
  • Gene Expression Regulation, Developmental*
  • Gene Library
  • Molecular Sequence Data
  • Protein-Serine-Threonine Kinases/biosynthesis*
  • Protein-Serine-Threonine Kinases/genetics
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Sequence Alignment
  • Transcription, Genetic
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins
  • Zygote/enzymology
PubMed: 12031503 Full text @ BBA Gene Structure and Expression
Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid -blastula transition (MBT) and throughout embryonic development.