PUBLICATION
Sequence and spatial expression of zebrafish (Danio rerio) alphaA-crystallin
- Authors
- Runkle, S., Hill, J., Kantorow, M., Horwitz, J., and Posner, M.
- ID
- ZDB-PUB-020403-14
- Date
- 2002
- Source
- Molecular Vision 8: 45-50 (Journal)
- Registered Authors
- Posner, Mason
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Crystallins/genetics*
- Crystallins/isolation & purification
- Crystallins/metabolism
- Gene Expression Regulation
- Humans
- Lens, Crystalline/chemistry
- Lens, Crystalline/metabolism
- Molecular Sequence Data
- RNA, Messenger/analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Zebrafish/genetics*
- PubMed
- 11925526
Citation
Runkle, S., Hill, J., Kantorow, M., Horwitz, J., and Posner, M. (2002) Sequence and spatial expression of zebrafish (Danio rerio) alphaA-crystallin. Molecular Vision. 8:45-50.
Abstract
To determine the nucleotide sequence, amino acid sequence and tissue specificity of zebrafish alphaA-crystallin. METHODS: RACE, both 3' and 5', was used to clone the zebrafish alphaA-crystallin gene. The peptide sequence of the encoded protein was deduced and compared to cavefish, shark, amphibian, bird and human orthologues using the CLUSTAL W algorithm. alphaA-crystallin transcript was evaluated in brain, heart, lens, liver, skeletal muscle/skin, and spleen by semi-quantitative RT-PCR. RESULTS: The 173 amino acid sequence of zebrafish alphaA-crystallin was determined to be 73% and 86% similar to its human and cavefish orthologues, respectively. We detected high expression of zebrafish alphaA-crystallin in the lens and very low expression in liver and spleen. CONCLUSIONS: Few amino acids identified as being functionally important to chaperone function differ between zebrafish and mammalian alphaA-crystallin. The expression of alphaA-crystallin is mainly confined to the lens in both taxa. These data suggest that zebrafish alphaA-crystallin plays a physiologically limited role outside of the zebrafish lens, similar to its mammalian orthologues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping