Cadherin-4 expression in the zebrafish central nervous system and regulation by ventral midline signaling
- Liu, Q., Marrs, J.A., Chuang, J.C., and Raymond, P.A.
- Brain research. Developmental brain research 131(1-2): 17-29 (Journal)
- Registered Authors
- Liu, Qin, Marrs, James A., Raymond, Pamela
- zebrafish embryo; cell adhesion molecule; transcription factor
- MeSH Terms
- Amino Acid Sequence
- Central Nervous System/embryology*
- Central Nervous System/physiology*
- Eye Proteins
- Gene Expression Regulation, Developmental/physiology*
- Hedgehog Proteins
- Homeodomain Proteins/genetics
- In Situ Hybridization
- Molecular Sequence Data
- Paired Box Transcription Factors
- RNA, Messenger/analysis
- RNA, Messenger/pharmacology
- Repressor Proteins
- Signal Transduction/physiology*
- Spinal Cord/embryology
- Spinal Cord/physiology
- Transcription Factors/genetics
- 11718832 Full text @ Brain Res. Dev. Brain Res.
Liu, Q., Marrs, J.A., Chuang, J.C., and Raymond, P.A. (2001) Cadherin-4 expression in the zebrafish central nervous system and regulation by ventral midline signaling. Brain research. Developmental brain research. 131(1-2):17-29.
In this study we show that zebrafish cadherin-4 (R-cadherin) transcript (cad4) and protein are expressed in several defined regions in the embryonic forebrain and in distinctive clusters in the hindbrain and spinal cord. This is the first report of a segmental pattern of expression of cadherin-4 in the hindbrain and spinal cord. Expression domains of cadherin-4 transcript and protein were compared with that of pax6.1. In the forebrain of zebrafish embryos, cad4 and pax6.1 expression domains overlapped extensively, although they were not completely coincident. Injection of pax6.1 mRNA resulted in an increase in cad4 expression, whereas overexpression of sonic hedgehog (shh), a midline signaling molecule that reduces pax6.1 expression, caused a reduction in cad4 expression throughout the brain. cad4 expression was increased in both forebrain and hindbrain in cyclops mutant embryos, which have a defect in midline signaling and an enlarged expression domain of pax6.1. These results suggest that zebrafish cadherin-4 may play a role in organization of neuronal architecture throughout the neural axis, and that its expression is regulated by a ventral midline signaling pathway that involves shh and pax6.1.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes