PUBLICATION
Genetic analysis of digestive physiology using fluorescent phospholipid reporters
- Authors
- Farber, S.A., Pack, M., Ho, S.Y., Johnson, I.D., Wagner, D.S., Dosch, R., Mullins, M.C., Hendrickson, H.S., Hendrickson, E.K., and Halpern, M.E.
- ID
- ZDB-PUB-010522-2
- Date
- 2001
- Source
- Science (New York, N.Y.) 292(5520): 1385-1388 (Journal)
- Registered Authors
- Dosch, Roland, Farber, Steven, Halpern, Marnie E., Ho, Shiu-Ying, Mullins, Mary C., Pack, Michael, Wagner, Daniel
- Keywords
- none
- MeSH Terms
-
- Phospholipids/metabolism*
- Heptanoic Acids/pharmacology
- Digestive System Physiological Phenomena*/drug effects
- Boron Compounds/metabolism
- Larva/drug effects
- Larva/metabolism
- Animals
- Anticholesteremic Agents/pharmacology
- Bile Acids and Salts/pharmacology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish/physiology
- Cholesterol/metabolism
- Fluorescent Dyes/metabolism*
- Microscopy, Video
- Atorvastatin
- Mutation/genetics
- Gallbladder/drug effects
- Gallbladder/metabolism
- Microscopy, Fluorescence
- Signal Transduction/drug effects
- Mice
- Digestive System/drug effects
- Digestive System/metabolism*
- Digestive System/pathology
- Lipase/metabolism
- Pyrroles/pharmacology
- PubMed
- 11359013 Full text @ Science
Citation
Farber, S.A., Pack, M., Ho, S.Y., Johnson, I.D., Wagner, D.S., Dosch, R., Mullins, M.C., Hendrickson, H.S., Hendrickson, E.K., and Halpern, M.E. (2001) Genetic analysis of digestive physiology using fluorescent phospholipid reporters. Science (New York, N.Y.). 292(5520):1385-1388.
Abstract
Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping