ZFIN ID: ZDB-PUB-010522-2
Genetic analysis of digestive physiology using fluorescent phospholipid reporters
Farber, S.A., Pack, M., Ho, S.Y., Johnson, I.D., Wagner, D.S., Dosch, R., Mullins, M.C., Hendrickson, H.S., Hendrickson, E.K., and Halpern, M.E.
Date: 2001
Source: Science (New York, N.Y.)   292(5520): 1385-1388 (Journal)
Registered Authors: Dosch, Roland, Farber, Steven, Halpern, Marnie E., Ho, Shiu-Ying, Mullins, Mary C., Pack, Michael, Wagner, Daniel
Keywords: none
MeSH Terms:
  • Animals
  • Anticholesteremic Agents/pharmacology
  • Atorvastatin
  • Bile Acids and Salts/pharmacology
  • Boron Compounds/metabolism
  • Cholesterol/metabolism
  • Digestive System/drug effects
  • Digestive System/metabolism*
  • Digestive System/pathology
  • Digestive System Physiological Phenomena*/drug effects
  • Fluorescent Dyes/metabolism*
  • Gallbladder/drug effects
  • Gallbladder/metabolism
  • Heptanoic Acids/pharmacology
  • Larva/drug effects
  • Larva/metabolism
  • Lipase/metabolism
  • Mice
  • Microscopy, Fluorescence
  • Microscopy, Video
  • Mutation/genetics
  • Phospholipids/metabolism*
  • Pyrroles/pharmacology
  • Signal Transduction/drug effects
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish/physiology
PubMed: 11359013 Full text @ Science
Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.