PUBLICATION
Two-color detection of mRNA transcript localizations in fish and fly embryos using alkaline phosphatase and beta-galactosidase conjugated antibodies
- Authors
- Hauptmann, G.
- ID
- ZDB-PUB-010404-13
- Date
- 1999
- Source
- Development genes and evolution 209(5): 317-321 (Journal)
- Registered Authors
- Hauptmann, Giselbert
- Keywords
- none
- MeSH Terms
-
- Immunohistochemistry
- Fishes/embryology*
- RNA, Messenger/genetics*
- beta-Galactosidase/immunology*
- Drosophila/embryology*
- Alkaline Phosphatase/immunology*
- Embryo, Nonmammalian/metabolism
- Nucleic Acid Hybridization
- Animals
- PubMed
- 11252185 Full text @ Dev. Genes Evol.
Citation
Hauptmann, G. (1999) Two-color detection of mRNA transcript localizations in fish and fly embryos using alkaline phosphatase and beta-galactosidase conjugated antibodies. Development genes and evolution. 209(5):317-321.
Abstract
A fast and simplified two-color in situ hybridization procedure for the simultaneous detection of two different mRNAs in whole-mounted zebrafish and Drosophila embryos is presented. Transcript distributions are detected in a single incubation step using a mixture of alkaline phosphatase and beta-galactosidase coupled antibodies. The different transcripts are visualized in contrasting colors by the use of beta-galactosidase substrates that develop color precipitates (magenta, blue) easily distinguishable from those of the standard alkaline phosphatase substrates. This protocol can be followed by standard immunohistochemistry to detect the expression of a third gene (at the protein level) in a third color.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping