ZFIN ID: ZDB-PERS-970128-5
Hauptmann, Giselbert
Email: giselbert.hauptmann@su.se
URL:
Affiliation: Hauptmann Lab
Address: Stockholm University Department of Molecular Biosciences The Wenner-Gren Institute Svante Arrhenius väg 20C Stockholm, S-10691 Sweden
Country: Sweden
Phone:
Fax:
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BIOGRAPHY AND RESEARCH INTERESTS
In situ mRNA detection and visualization in neural tissue

Evolution and development of vertebrate forebrain organization

Development and function of neuroendocrine hypothalamus

Development and function of corticotropin-releasing-hormone neuronal
systems


PUBLICATIONS
Hauptmann, G., Lauter, G., Söll, I. (2016) Detection and signal amplification in Zebrafish RNA FISH. Methods (San Diego, Calif.). 98:50-9
Lauter, G., Söll, I., and Hauptmann, G. (2014) Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification. Meth. Mol. Biol.. 1082:175-185
Lauter, G., Söll, I., and Hauptmann, G. (2013) Molecular characterization of prosomeric and intraprosomeric subdivisions of the embryonic zebrafish diencephalon. The Journal of comparative neurology. 521(5):1093-1118
Bräutigam, L., Schütte, L.D., Godoy, J.R., Prozorovski, T., Gellert, M., Hauptmann, G., Holmgren, A., Lillig, C.H., and Berndt, C. (2011) Vertebrate-specific glutaredoxin is essential for brain development. Proc. Natl. Acad. Sci. USA. 108(51):20532-7
Lauter, G., Soll, I., and Hauptmann, G. (2011) Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems. BMC Developmental Biology. 11(1):43
Lauter, G., Söll, I., and Hauptmann, G. (2011) Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain. Neural Development. 6:10
Bräutigam, L., Hillmer, J.M., Söll, I., and Hauptmann, G. (2010) Localized expression of urocortin genes in the developing zebrafish brain. The Journal of comparative neurology. 518(15):2978-2995
Lee, S.L., Rouhi, P., Jensen, L.D., Zhang, D., Ji, H., Hauptmann, G., Ingham, P., and Cao, Y. (2009) Hypoxia-induced pathological angiogenesis mediates tumor cell dissemination, invasion, and metastasis in a zebrafish tumor model. Proc. Natl. Acad. Sci. USA. 106(46):19485-19490
Dahl Ejby Jensen, L., Cao, R., Hedlund, E.M., Söll, I., Lundberg, J.O., Hauptmann, G., Steffensen, J.F., and Cao, Y. (2009) Nitric oxide permits hypoxia-induced lymphatic perfusion by controlling arterial-lymphatic conduits in zebrafish and glass catfish. Proc. Natl. Acad. Sci. USA. 106(43):18408-18413
Gabriel, J.P., Mahmood, R., Kyriakatos, A., Söll, I., Hauptmann, G., Calabrese, R.L., and El Manira, A. (2009) Serotonergic modulation of locomotion in zebrafish: endogenous release and synaptic mechanisms. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(33):10387-10395
Cao, R., Jensen, L.D., Söll, I., Hauptmann, G., and Cao, Y. (2008) Hypoxia-induced retinal angiogenesis in zebrafish as a model to study retinopathy. PLoS One. 3(7):e2748
Archer, A., Lauter, G., Hauptmann, G., Mode, A., and Gustafsson, J.A. (2008) Transcriptional activity and developmental expression of liver X receptor (lxr) in Zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 237(4):1090-1098
Gabriel, J.P., Mahmood, R., Walter, A.M., Kyriakatos, A., Hauptmann, G., Calabrese, R.L., and El Manira, A. (2008) Locomotor pattern in the adult zebrafish spinal cord in vitro. Journal of neurophysiology. 99(1):37-48
Chandrasekar, G., Lauter, G., and Hauptmann, G. (2007) Distribution of corticotropin-releasing hormone in the developing zebrafish brain. The Journal of comparative neurology. 505(4):337-351
Kitambi, S.S., and Hauptmann, G. (2007) The zebrafish orphan nuclear receptor genes nr2e1 and nr2e3 are expressed in developing eye and forebrain. Gene Expression Patterns. 7(4):521-528
Holzschuh, J., Hauptmann, G., and Driever, W. (2003) Genetic analysis of the roles of Hh, FGF8, and nodal signaling during catecholaminergic system development in the zebrafish brain. The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(13):5507-5519
Hauptmann, G., Belting, H.-G., Wolke, U., Lunde, K., Söll, I., Abdelilah-Seyfried, S., Prince, V., and Driever, W. (2002) spiel ohne grenzen/pou2 is required for zebrafish hindbrain segmentation. Development (Cambridge, England). 129(7):1645-1655
Hauptmann, G., Söll, I., and Gerster, T. (2002) The early embryonic zebrafish forebrain is subdivided into molecularly distinct transverse and longitudinal domains. Brain research bulletin. 57(3-4):371-375
Belting, H.G., Hauptmann, G., Meyer, D., Abdelilah-Seyfried, S., Chitnis, A., Eschbach, C., Söll, I., Thisse, C., Thisse, B., Artinger, K., Lunde, K., and Driever, W. (2001) spiel ohne grenzen/pou2 is required during establishment of the zebrafish midbrain-hindbrain boundary organizer. Development (Cambridge, England). 128(21):4165-4176
Hauptmann, G. (2001) One-, two-, and three-color whole-mount in situ hybridization to Drosophila embryos. Methods (San Diego, Calif.). 23(4):359-372
Hauptmann, G. and Gerster, T. (2000) Combinatorial expression of zebrafish brn-1- and brn-2-related POU genes in the embryonic brain, pronephric primordium, and pharyngeal arches. Developmental dynamics : an official publication of the American Association of Anatomists. 218(2):345-358
Hauptmann, G. and Gerster, T. (2000) Regulatory gene expression patterns reveal transverse and longitudinal subdivisions of the embryonic zebrafish forebrain. Mechanisms of Development. 91(1-2):105-118
Hauptmann, G. and Gerster, T. (2000) Multicolor whole-mount in situ hybridization. In Developmental Biology Protocols, Vol III, R.S. Tuan and C.W. Lo, eds., Totowa, NJ: Humana Press, Meth. Mol. Biol.. 137(14):139-148
Hauptmann, G. (1999) Two-color detection of mRNA transcript localizations in fish and fly embryos using alkaline phosphatase and beta-galactosidase conjugated antibodies. Development genes and evolution. 209(5):317-321
Spaniol, P., Bornmann, C., Hauptmann, G., and Gerster, T. (1996) Class III POU genes of zebrafish are predominantly expressed in the central nervous system. Nucleic acids research. 24(24):4874-4881
Hauptmann, G. and Gerster, T. (1996) Complex expression of the zp-50 pou gene in the embryonic zebrafish brain is altered by overexpression of sonic hedgehog. Development (Cambridge, England). 122(6):1769-1780
Hauptmann, G. and Gerster, T. (1995) Pou-2 — a zebrafish gene active during cleavage stages and in the early hindbrain. Mechanisms of Development. 51(1):127-138
Hauptmann, G. and Gerster, T. (1994) Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. Trends in genetics : TIG. 10(8):266

NON-ZEBRAFISH PUBLICATIONS
Hauptmann, G. & Gerster, T.
"Multicolor whole-mount in situ hybridization to Drosophila embryos"
Dev. Genes Evol. 1996 Dec (206): 292-295
ABSTRACT
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin,
respectively, this protocol allows the detection in three colors of RNAs derived from three different genes. Hybridized probes are detected by consecutive
staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by
low pH washes.