PUBLICATION
Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene
- Authors
- Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J.
- ID
- ZDB-PUB-010220-9
- Date
- 2001
- Source
- Genesis (New York, N.Y. : 2000) 29(1): 22-35 (Journal)
- Registered Authors
- Chen, Yau-Hung, Lee, Wen-Chih, Tsai, Huai-Jen
- Keywords
- fish; microinjection; molecular structure; muscle-specificity; somitogenesis; transgenic fish
- MeSH Terms
-
- Amino Acid Sequence
- DNA-Binding Proteins*
- Polymerase Chain Reaction
- Animals, Genetically Modified
- Myogenic Regulatory Factor 5
- Animals
- Molecular Sequence Data
- Promoter Regions, Genetic*
- Sequence Homology, Amino Acid
- Exons
- Green Fluorescent Proteins
- RNA, Messenger/biosynthesis
- Trans-Activators*
- Transcription Factors/genetics*
- Transcription, Genetic
- Genetic Vectors
- Muscle Proteins/genetics*
- Muscle Proteins/metabolism
- In Situ Hybridization
- DNA Primers/chemistry
- Introns
- Luminescent Proteins/metabolism
- Cloning, Molecular
- Base Sequence
- Genes, Regulator
- Gene Expression
- Zebrafish/genetics*
- PubMed
- 11135459 Full text @ Genesis
Citation
Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J. (2001) Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene. Genesis (New York, N.Y. : 2000). 29(1):22-35.
Abstract
Summary: We isolated a 1,438 bp cDNA fragment that encoded Myf-5 myogenic factor of zebrafish. The deduced amino acid contained 237 residues, including the basic helix-loop-helix domain that is conserved in all known Myf-5. The zebrafish myf-5 transcripts were first detectable at 7.5 hpf, increased substantially until 16 hpf, and then declined gradually to an undetectable level by 26 hpf. During somitogenesis, zebrafish myf-5 transcripts were distributed mainly in the somites and segmental plates. Prominent signals occurred transiently in adaxial cells in two parallel rows but did not extend beyond the positive-signal somites. Various lengths of upstream region of zebrafish myf-5 fused with EGFP gene were used to carry out transgenic analysis. Results showed that a small, 82 bp (nucleotide positions from -82 to -1), regulatory cassette is sufficient to control the somite- and stage-specific expression of zebrafish myf-5 during early development. genesis 29:22-35, 2001.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping