PUBLICATION

Slow muscle induction by Hedgehog signalling in vitro

Authors
Norris, W., Neyt, C., Ingham, P.W., and Currie, P.D.
ID
ZDB-PUB-000824-11
Date
2000
Source
Journal of Cell Science   113(Pt.15): 2695-2703 (Journal)
Registered Authors
Currie, Peter D., Ingham, Philip, Neyt, Christine
Keywords
Sonic Hedgehog; zebrafish; myoblast; cell fate
MeSH Terms
  • Animals
  • Cell Differentiation/physiology
  • Cells, Cultured
  • Embryonic Induction/physiology
  • Hedgehog Proteins
  • Heterotrimeric GTP-Binding Proteins/physiology
  • In Vitro Techniques
  • Muscle Fibers, Slow-Twitch/cytology*
  • Muscle Fibers, Slow-Twitch/physiology*
  • Muscle, Skeletal/cytology
  • Muscle, Skeletal/embryology
  • Proteins/physiology*
  • Signal Transduction/physiology*
  • Trans-Activators*
  • Zebrafish
PubMed
10893185 Full text @ J. Cell Sci.
Abstract
Muscles are composed of several fibre types, the precise combination of which determines muscle function. Whereas neonatal and adult fibre type is influenced by a number of extrinsic factors, such as neural input and muscle load, there is little knowledge of how muscle cells are initially determined in the early embryo. In the zebrafish, fibres of the slow twitch class arise from precociously specified myoblasts that lie close to the midline whereas the remainder of the myotome differentiates as fast myosin expressing muscle. In vivo evidence has suggested the Sonic Hedgehog glycoprotein, secreted from the notochord, controls the formation of slow twitch and fast twitch muscle fates. Here we describe an in vitro culture system that we have developed to test directly the ability of zebrafish myoblasts to respond to exogenous Sonic Hedgehog peptide. We find that Sonic Hedgehog peptide can control the binary cell fate choice of embryonic zebrafish myoblasts in vitro. We have also used this culture system to assay the relative activities of different Hedgehog-family proteins and to investigate the possible involvement of heterotrimeric G-proteins in Hedgehog signal transduction.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping