PUBLICATION
Regulatory gene expression patterns reveal transverse and longitudinal subdivisions of the embryonic zebrafish forebrain
- Authors
- Hauptmann, G. and Gerster, T.
- ID
- ZDB-PUB-000309-28
- Date
- 2000
- Source
- Mechanisms of Development 91(1-2): 105-118 (Journal)
- Registered Authors
- Gerster, Thomas, Hauptmann, Giselbert
- Keywords
- forebrain; diencephalon; prosomere; neuromere; longitudinal brain axis; alar/basal boundary; floor plate; roof plate; alar plate; basal plate; two-color whole-mount; in situ hybridization
- MeSH Terms
-
- Animals
- Axis, Cervical Vertebra
- Body Patterning
- DNA-Binding Proteins/genetics
- Eye Proteins
- Gene Expression Regulation, Developmental*
- Hedgehog Proteins
- Homeodomain Proteins/genetics
- Nerve Tissue Proteins/genetics
- Otx Transcription Factors
- PAX2 Transcription Factor
- POU Domain Factors
- Paired Box Transcription Factors
- Prosencephalon/embryology*
- Proteins/genetics
- Repressor Proteins
- Trans-Activators/genetics
- Transcription Factors/genetics
- Zebrafish/embryology*
- Zebrafish Proteins*
- PubMed
- 10704836 Full text @ Mech. Dev.
Citation
Hauptmann, G. and Gerster, T. (2000) Regulatory gene expression patterns reveal transverse and longitudinal subdivisions of the embryonic zebrafish forebrain. Mechanisms of Development. 91(1-2):105-118.
Abstract
To shed light on the organization of the rostral embryonic brain of a lower vertebrate, we have directly compared the expression patterns of dlx, fgf, hh, hlx, otx, pax, POU, winged helix and wnt gene family members in the fore- and midbrain of the zebrafish. We show that the analyzed genes are expressed in distinct transverse and longitudinal domains and share expression boundaries at stereotypic positions within the fore- and midbrain. Some of these shared expression boundaries coincide with morphological landmarks like the pathways of primary axon tracts. We identified a series of eight transverse diencephalic domains suggestive of neuromeric subdivisions within the rostral brain. In addition, we identified four molecularly distinct longitudinal subdivisions and provide evidence for a strong bending of the longitudinal rostral brain axis at the cephalic flexure. Our data suggest a strong conservation of early forebrain organization between lower and higher vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping