PUBLICATION
Pleiotropic effects of zebrafish protein-tyrosine phosphatase-1B on early embryonic development
- Authors
- van der Sar, A.M., de Fockert, J., Betist, M., Zivkovic, D., and den Hertog, J.
- ID
- ZDB-PUB-000309-18
- Date
- 1999
- Source
- The International journal of developmental biology 43(8): 785-794 (Journal)
- Registered Authors
- de Fockert, Jaco, den Hertog, Jeroen, Jongejan-Zivkovic, Dana
- Keywords
- none
- MeSH Terms
-
- Base Sequence
- Endoplasmic Reticulum/enzymology
- Gene Expression Regulation, Developmental
- Animals
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Cloning, Molecular
- Sequence Homology, Amino Acid
- Mutagenesis, Site-Directed
- In Situ Hybridization
- Humans
- Protein Tyrosine Phosphatases/genetics
- Protein Tyrosine Phosphatases/metabolism*
- Phenotype
- Amino Acid Sequence
- Female
- Molecular Sequence Data
- DNA Primers/genetics
- PubMed
- 10707902
Citation
van der Sar, A.M., de Fockert, J., Betist, M., Zivkovic, D., and den Hertog, J. (1999) Pleiotropic effects of zebrafish protein-tyrosine phosphatase-1B on early embryonic development. The International journal of developmental biology. 43(8):785-794.
Abstract
Protein tyrosine phosphorylation is an important mechanism of eukaryotic cell signalling which is regulated by protein-tyrosine kinases and protein-tyrosine phosphatases. Here we report the molecular cloning of the first zebrafish protein-tyrosine phosphatase, zf-PTP-1B, the homologue of human PTP-1B. Zf-PTP-1B was catalytically active and localised to the endoplasmic reticulum, like human PTP-1B. Zf-PTP-1B was maternally expressed in zebrafish embryos, and low ubiquitous expression was detected up to day 7 of development. Microinjection of zf-PTP-1B RNA induced pleiotropic, but reproducible developmental defects. Evaluation of the live embryos at 24 h post fertilisation indicated that zf-PTP-1B induced defects in somite formation. The phenotype was dependent on protein-tyrosine phosphatase activity of zf-PTP-1B, since embryos injected with catalytically inactive zf-PTP-1B-C213S developed normally. Co-injection of wild type and inactive zf-PTP-1B led to a rescue of the zf-PTP-1B-induced phenotype, suggesting that zf-PTP-1B-C213S had dominant negative activity. The zf-PTP-1B-induced phenotype suggests that proper tyrosine phosphorylation of key proteins is essential for early development, most notably somitogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping