Morpholino

MO1-fgf22

ID

ZDB-MRPHLNO-130911-1

Name

MO1-fgf22

Previous Names

fgf22 exon 2/intron 2 spliceblocking MO1 (1)

Target

fgf22 (1)

Sequence

5' - ATGCGATGTACCTACCGATCCGAAAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Splice-blocking MO.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-fgf22

Expressed Gene	Anatomy	Figures
bmp5	roof plate midbrain region	Fig. 5 from Miyake et al., 2013
en2a	midbrain postero-ventral region midbrain hindbrain boundary	Fig. 4 from Miyake et al., 2013
fgf3	(not) midbrain hindbrain boundary dorsal-most region	Fig. 8 from Miyake et al., 2013
fgf8a	midbrain hindbrain boundary	Fig. 8 from Miyake et al., 2013
her5	midbrain hindbrain boundary	Fig. 4 from Miyake et al., 2013
lmx1ba	roof plate midbrain region	Fig. 5 from Miyake et al., 2013
meis2a	midbrain	Fig. 6 from Miyake et al., 2013
msx1b	roof plate midbrain region	Fig. 5 from Miyake et al., 2013
nkx6.2	midbrain	Fig. 6 from Miyake et al., 2013
otx2b	midbrain (not) optic tectum dorsal-most region	Fig. 6 from Miyake et al., 2013
pax2a	midbrain hindbrain boundary	Fig. 4 from Miyake et al., 2013
pax7a	midbrain	Fig. 6 from Miyake et al., 2013
shha	midbrain	Fig. 7 from Miyake et al., 2013
wnt1	midbrain dorsal region midbrain hindbrain boundary	Fig. 4 , Fig. 5 from Miyake et al., 2013

Phenotype

Phenotype resulting from MO1-fgf22

Phenotype	Fish	Figures
cell proliferation in midbrain decreased occurrence, abnormal	WT + MO1-fgf22	Fig. 3 from Miyake et al., 2013
forebrain morphology, abnormal	WT + MO1-fgf22	Fig. 2 from Miyake et al., 2013
forebrain development disrupted, abnormal	WT + MO1-fgf22 + MO4-tp53	Fig. 2 from Miyake et al., 2013
midbrain morphology, abnormal	WT + MO1-fgf22	Fig. 2 from Miyake et al., 2013
midbrain development disrupted, abnormal	WT + MO1-fgf22 + MO4-tp53	Fig. 2 , Fig. 5 from Miyake et al., 2013
midbrain hindbrain boundary morphology, abnormal	WT + MO1-fgf22	Fig. 2 from Miyake et al., 2013
midbrain-hindbrain boundary development disrupted, abnormal	WT + MO1-fgf22 + MO4-tp53	Fig. 2 from Miyake et al., 2013
roof plate formation disrupted, abnormal	WT + MO1-fgf22	Fig. 4 from Miyake et al., 2013
roof plate midbrain region increased width, abnormal	WT + MO1-fgf22	Fig. 4 from Miyake et al., 2013

Phenotype of all Fish created by or utilizing MO1-fgf22

Phenotype	Fish	Conditions	Figures
forebrain development disrupted, abnormal	WT + MO1-fgf22	standard conditions	Fig. 2 from Miyake et al., 2013
roof plate midbrain region increased width, abnormal	WT + MO1-fgf22	standard conditions	Fig. 4 from Miyake et al., 2013
midbrain-hindbrain boundary development disrupted, abnormal	WT + MO1-fgf22	standard conditions	Fig. 2 from Miyake et al., 2013
midbrain development disrupted, abnormal	WT + MO1-fgf22	standard conditions	Fig. 2 , Fig. 5 from Miyake et al., 2013
midbrain morphology, abnormal	WT + MO1-fgf22	standard conditions	Fig. 2 from Miyake et al., 2013
forebrain morphology, abnormal	WT + MO1-fgf22	standard conditions	Fig. 2 from Miyake et al., 2013
roof plate formation disrupted, abnormal	WT + MO1-fgf22	standard conditions	Fig. 4 from Miyake et al., 2013
cell proliferation in midbrain decreased occurrence, abnormal	WT + MO1-fgf22	standard conditions	Fig. 3 from Miyake et al., 2013
midbrain hindbrain boundary morphology, abnormal	WT + MO1-fgf22	standard conditions	Fig. 2 from Miyake et al., 2013
forebrain morphology, abnormal	WT + MO1-fgf22 + MO4-tp53	standard conditions	Fig. 2 from Miyake et al., 2013
forebrain development disrupted, abnormal	WT + MO1-fgf22 + MO4-tp53	standard conditions	Fig. 2 from Miyake et al., 2013
midbrain-hindbrain boundary development disrupted, abnormal	WT + MO1-fgf22 + MO4-tp53	standard conditions	Fig. 2 from Miyake et al., 2013
midbrain development disrupted, abnormal	WT + MO1-fgf22 + MO4-tp53	standard conditions	Fig. 2 from Miyake et al., 2013
midbrain hindbrain boundary morphology, abnormal	WT + MO1-fgf22 + MO4-tp53	standard conditions	Fig. 2 from Miyake et al., 2013
midbrain morphology, abnormal	WT + MO1-fgf22 + MO4-tp53	standard conditions	Fig. 2 from Miyake et al., 2013

Citations