ZFIN Lab: Nicolson Lab

ZFIN ID: ZDB-LAB-001113-2

Nicolson Lab

PI/Director:	Nicolson, Teresa
Contact Person:	Nicolson, Teresa
Email:	tnicolso@stanford.edu
URL:	http://nicolsonlab.stanford.edu/
Address:
Country:	United States
Phone:
Fax:
Line Designation:	st

GENOMIC FEATURES ORIGINATING FROM THIS LAB

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STATEMENT OF RESEARCH INTERESTS

Defects in sensory hair cells are thought to account for most forms of hereditary deafness. We aim to identify the components involved in hair-cell function by using a genetic approach. By screening over 7000 genomes (Tuebingen Screens I & II), we have identified a total of 24 genes that are required for balance and hearing in zebrafish. These circler mutants are analogous to the mouse shaker-waltzer mutants and can serve as alternative models for the study of human deafness. We are currently developing new ways of analyzing our mutants such stably expressing hair-cell specific markers, creating behavioral tests for auditory and vestibular function, and measuring physiological responses in hair cells and acousticolateralis nerves.

LAB MEMBERS

Gao, Yan Post-Doc	Shipman, Anna Post-Doc	Sun, Peng Post-Doc
Brodo-Abo, Sivan Research Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Sun, P., Smith, E., Nicolson, T. (2023) Transmembrane channel-like (Tmc) subunits contribute to frequency sensitivity in the zebrafish utricle. The Journal of neuroscience : the official journal of the Society for Neuroscience. 44(1):

Gao, Y., Khan, Y.A., Mo, W., White, K.I., Perkins, M., Pfuetzner, R.A., Trapani, J.G., Brunger, A.T., Nicolson, T. (2023) Sensory deficit screen identifies nsf mutation that differentially affects SNARE recycling and quality control. Cell Reports. 42:112345112345

Gao, Y., Nicolson, T. (2021) Temporal Vestibular Deficits in synaptojanin 1 (synj1) Mutants. Frontiers in molecular neuroscience. 13:604189

Smith, E.T., Pacentine, I., Shipman, A., Hill, M., Nicolson, T. (2020) Disruption of tmc1/2a/2b genes in zebrafish reveals subunit requirements in subtypes of inner ear hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 40(23):4457-4468

Gao, Y., Yang, X., Wang, Z., Zhong, Z., Hu, Y., Wang, Y. (2020) Supramolecular Nano-Encapsulation of Anabasine Reduced Its Developmental Toxicity in Zebrafish. Frontiers in chemistry. 8:134

Erickson, T., Pacentine, I.V., Venuto, A., Clemens, R., Nicolson, T. (2020) The lhfpl5 Ohnologs lhfpl5a and lhfpl5b Are Required for Mechanotransduction in Distinct Populations of Sensory Hair Cells in Zebrafish. Frontiers in molecular neuroscience. 12:320

Gao, Y., Lee, H., Kwon, O.K., Tan, M., Kim, K.T., Lee, S. (2019) Global proteomic analysis of lysine succinylation in zebrafish (Danio rerio). Journal of Proteome Research. 18(10):3762-3769

Pacentine, I.V., Nicolson, T. (2019) Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells. PLoS Genetics. 15:e1007635

Han, S., Liu, X., Xie, S., Gao, M., Liu, F., Yu, S., Sun, P., Wang, C., Archacki, S., Lu, Z., Hu, X., Qin, Y., Qu, Z., Huang, Y., Lv, Y., Tu, J., Li, J., Yimer, T.A., Jiang, T., Tang, Z., Luo, D., Chen, F., Liu, M. (2018) Knockout of ush2a gene in zebrafish causes hearing impairment and late onset rod-cone dystrophy. Human genetics. 137(10):779-794

Sun, P., Zhang, Y., Zhao, F., Wu, J.P., Pun, S.H., Peng, C., Du, M., Vai, M.I., Liu, D., Chen, F. (2018) An Assay for Systematically Quantifying the Vestibulo-Ocular Reflex to Assess Vestibular Function in Zebrafish Larvae. Frontiers in Cellular Neuroscience. 12:257

Chandrasekhar, A., Guo, S., Masai, I., Nicolson, T., Wu, C.F. (2017) Zebrafish: from genes and neurons to circuits, behavior and disease. Journal of neurogenetics. 31:59-60

Nicolson, T. (2017) The genetics of hair-cell function in zebrafish. Journal of neurogenetics. 31(3):102-112

Sheets, L., He, X.J., Olt, J., Schreck, M., Petralia, R.S., Wang, Y.X., Zhang, Q., Beirl, A., Nicolson, T., Marcotti, W., Trapani, J.G., Kindt, K.S. (2017) Enlargement of ribbons in zebrafish hair cells increases calcium currents, but disrupts afferent spontaneous activity and timing of stimulus onset. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(26):6299-6313

Erickson, T., Morgan, C.P., Olt, J., Hardy, K., Busch-Nentwich, E.M., Maeda, R., Clemens-Grisham, R., Krey, J.F., Nechiporuk, A.V., Barr-Gillespie, P.G., Marcotti, W., Nicolson, T. (2017) Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt). eLIFE. 6

Maeda, R., Pacentine, I.V., Erickson, T., Nicolson, T. (2017) Functional analysis of the transmembrane and cytoplasmic domains of Pcdh15a in zebrafish hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(12):3231-3245

Wang, C., Zhong, Z., Sun, P., Zhong, H., Li, H., Chen, F. (2017) Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses. Neural Plasticity. 2017:8283075

Erickson, T., Nicolson, T. (2016) Cell type-specific transcriptomic analysis by thiouracil tagging in zebrafish. Methods in cell biology. 135:309-28

Toro, C., Trapani, J.G., Pacentine, I., Maeda, R., Sheets, L., Mo, W., Nicolson, T. (2015) Dopamine Modulates the Activity of Sensory Hair Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(50):16494-16503

Nicolson, T. (2015) Ribbon Synapses in Zebrafish Hair Cells. Hearing Research. 330(Pt B):170-7

Erickson, T., Nicolson, T. (2015) Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish. BMC Genomics. 16:842

Sheets, L., Hagen, M.W., Nicolson, T. (2014) Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons. PLoS One. 9:e107256

Maeda, R., Kindt, K.S., Mo, W., Morgan, C.P., Erickson, T., Zhao, H., Clemens-Grisham, R., Barr-Gillespie, P.G., Nicolson, T. (2014) Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2. Proceedings of the National Academy of Sciences of the United States of America. 111(35):12907-12

Grisham, R.C., Kindt, K., Finger-Baier, K., Schmid, B., and Nicolson, T. (2013) Mutations in ap1b1 Cause Mistargeting of the Na(+)/K(+)-ATPase Pump in Sensory Hair Cells. PLoS One. 8(4):e60866

Kalueff, A.V., Gebhardt, M., Stewart, A.M., Cachat, J.M., Brimmer, M., Chawla, J.S., Craddock, C., Kyzar, E.J., Roth, A., Landsman, S., Gaikwad, S., Robinson, K., Baatrup, E., Tierney, K., Shamchuk, A., Norton, W., Miller, N., Nicolson, T., Braubach, O., Gilman, C.P., Pittman, J., Rosemberg, D.B., Gerlai, R., Echevarria, D., Lamb, E., Neuhauss, S.C., Weng, W., and Bally-Cuif, L. (2013) Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond. Zebrafish. 10(1):70-86

Sheets, L., Kindt, K.S., and Nicolson, T. (2012) Presynaptic CaV1.3 Channels Regulate Synaptic Ribbon Size and Are Required for Synaptic Maintenance in Sensory Hair Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(48):17273-17286

Obholzer, N., Swinburne, I.A., Schwab, E., Nechiporuk, A.V., Nicolson, T., and Megason, S.G. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing. Development (Cambridge, England). 139(22):4280-4290

Kindt, K.S., Finch, G., and Nicolson, T. (2012) Kinocilia mediate mechanosensitivity in developing zebrafish hair cells. Developmental Cell. 23(2):329-341

Einhorn, Z., Trapani, J.G., Liu, Q., and Nicolson, T. (2012) Rabconnectin3alpha promotes stable activity of the H+ pump on synaptic vesicles in hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(32):11144-11156

Glover, G., Mueller, K.P., Söllner, C., Neuhauss, S.C., and Nicolson, T. (2012) The Usher gene cadherin 23 is expressed in the zebrafish brain and a subset of retinal amacrine cells. Molecular Vision. 18:2309-2322

Sheets, L., Trapani, J.G., Mo, W., Obholzer, N., and Nicolson, T. (2011) Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England). 138(7):1309-1319

Trapani, J.G., and Nicolson, T. (2011) Mechanism of spontaneous activity in afferent neurons of the zebrafish lateral-line organ. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(5):1614-1623

Mo, W., and Nicolson, T. (2011) Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapses. PLoS One. 6(11):e27146

Mo, W., Chen, F., Nechiporuk, A., and Nicolson, T. (2010) Quantification of vestibular-induced eye movements in zebrafish larvae. BMC Neuroscience. 11:110

Wright, M.A., Mo, W., Nicolson, T., and Ribera, A.B. (2010) In vivo evidence for transdifferentiation of peripheral neurons. Development (Cambridge, England). 137(18):3047-3056

Trapani, J.G., and Nicolson, T. (2010) Chapter 8 - Physiological recordings from zebrafish lateral-line hair cells and afferent neurons. Methods in cell biology. 100:219-231

Trapani, J.G., Obholzer, N., Mo, W., Brockerhoff, S.E., and Nicolson, T. (2009) Synaptojanin1 is required for temporal fidelity of synaptic transmission in hair cells. PLoS Genetics. 5(5):e1000480

Obholzer, N., Wolfson, S., Trapani, J.G., Mo, W., Nechiporuk, A., Busch-Nentwich, E., Seiler, C., Sidi, S., Söllner, C., Duncan, R.N., Boehland, A., and Nicolson, T. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(9):2110-2118

Geisler, R., Rauch, G.J., Geiger-Rudolph, S., Albrecht, A., van Bebber, F., Berger, A., Busch-Nentwich, E., Dahm, R., Dekens, M.P., Dooley, C., Elli, A.F.,Gehring, I., Geiger, H., Geisler, M., Glaser, S., Holley, S., Huber, M., Kerr, A., Kirn, A., Knirsch, M., Konantz, M., Kuchler, A.M., Maderspacher, F., Neuhauss, S.C., Nicolson, T., Ober, E.A., Praeg, E., Ray, R., Rentzsch, B., Rick, J.M., Rief, E., Schauerte, H.E., Schepp, C.P., Schonberger, U., Schonthaler, H.B., Seiler, C., Sidi, S., Söllner, C., Wehner, A., Weiler, C., Nüsslein-Volhard, C. (2007) Large-scale mapping of mutations affecting zebrafish development. BMC Genomics. 8(1):11

Söllner, C., and Nicolson, T. (2005) The Zebrafish as a Genetic Model to Study Otolith Formation. Biomineralization (2nd Edition). :229-242

Seiler, C., Finger-Baier, K.C., Rinner, O., Makhankov, Y.V., Schwarz, H., Neuhauss, S.C., and Nicolson, T. (2005) Duplicated genes with split functions: independent roles of protocadherin15 orthologues in zebrafish hearing and vision. Development (Cambridge, England). 132(3):615-623

Nicolson, T. (2005) The genetics of hearing and balance in zebrafish. Annual review of genetics. 39:9-22

Söllner, C., Schwarz, H., Geisler, R., and Nicolson, T. (2004) Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish. Development genes and evolution. 214(12):582-590

Seiler, C., Ben-David, O., Sidi, S., Hendrich, O., Rusch, A., Burnside, B., Avraham, K.B., and Nicolson, T. (2004) Myosin VI is required for structural integrity of the apical surface of sensory hair cells in zebrafish. Developmental Biology. 272(2):328-338

Paukert, M., Sidi, S., Russell, C., Siba, M., Wilson, S.W., Nicolson, T., and Grunder, S. (2004) A family of acid-sensing ion channels (ASICs) from the zebrafish: Widespread expression in the central nervous system suggests a conserved role in neuronal communication. The Journal of biological chemistry. 279(18):18783-18791

Söllner, C., Rauch, G-J., Siemens, J., Geisler, R., Schuster, S.C., The Tübingen 2000 Screen Consortium, Müller, U., and Nicolson, T. (2004) Mutations in cadherin 23 affect tip links in zebrafish sensory hair cells. Nature. 428(6986):955-959

Sidi, S., Busch-Nentwich, E., Friedrich, R., Schoenberger, U., and Nicolson, T. (2004) gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses. The Journal of neuroscience : the official journal of the Society for Neuroscience. 24(17):4213-4223

Busch-Nentwich, E., Söllner, C., Roehl, H., and Nicolson, T. (2004) The deafness gene dfna5 is crucial for ugdh expression and HA production in the developing ear in zebrafish. Development (Cambridge, England). 131(4):943-951

Söllner, C., Burghammer, M., Busch-Nentwich, E., Berger, J., Schwarz, H., Riekel, C., and Nicolson, T. (2003) Control of crystal size and lattice formation by starmaker in otolith biomineralization. Science (New York, N.Y.). 302(5643):282-286

Concha, M.L., Russell, C., Regan, J.C., Tawk, M., Sidi, S., Gilmour, D.T., Kapsimali, M., Sumoy, L., Goldstone, K., Amaya, E., Kimelman, D., Nicolson, T., Gründer, S., Gomperts, M., Clarke, J.D.W., and Wilson, S.W. (2003) Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron. 39(3):423-438

Sidi, S., Friedrich, R.W., and Nicolson, T. (2003) NompC TRP channel required for vertebrate sensory hair cell mechanotransduction. Science (New York, N.Y.). 301(5629):96-99

Ernest, S., Rauch, G.J., Haffter, P., Geisler, R., Petit, C., and Nicolson, T. (2000) Mariner is defective in myosin VIIA: a zebrafish model for human hereditary deafness. Human molecular genetics. 9(14):2189-2196

Nicolson, T., Ernest, S., Rauch, J., Petit, C., Söllner, C., Seiler, C., and Busch, E. (2000) Isolation of the genes responsible for hearing disorders in zebrafish. The European journal of neuroscience. 12:316

Seiler, C. and Nicolson, T. (1999) Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants. Journal of neurobiology. 41(3):424-434

Geisler, R., Rauch, G.J., Baier, H., van Bebber, F., Brobeta, L., Dekens, M.P., Finger, K., Fricke, C., Gates, M.A., Geiger, H., Geiger-Rudolph, S., Gilmour, D., Glaser, S., Gnugge, L., Habeck, H., Hingst, K., Holley, S., Keenan, J., Kirn, A., Knaut, H., Lashkari, D., Maderspacher, F., Martyn, U., Neuhauss, S., Neumann, C., Nicolson, T., Pelegri, F., Ray, R., Rick, J.M., Roehl, H., Roeser, T., Schauerte, H.E., Schier, A.F., Schönberger, U., Schönthaler, H.-B., Schulte-Merker, S., Seydler, C., Talbot, W.S., Weiler, C., Nüsslein-Volhard, C., and Haffter, P. (1999) A radiation hybrid map of the zebrafish genome. Nature Genetics. 23(1):86-89

Nicolson, T., Rüsch, A., Friedrich, R.W., Granato, M., Ruppersberg, J.P., and Nüsslein-Volhard, C. (1998) Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutants. Neuron. 20:271-283