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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-971209-49
Nicolson, Teresa
Email: tnicolso@stanford.edu
URL: http://med.stanford.edu/ohns/research/labs/teresa-nicolson-lab.html
Affiliation: Nicolson Lab
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Country: United States
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BIOGRAPHY AND RESEARCH INTERESTS
Auditory/vestibular hair cells are excited by sound or head movements. Excitation occurs when hair bundles are deflected along the excitatory axis, placing tension on tips links that interconnect stereocilia. Tension is thought to mechanically gate cation- selective channels at the tips of stereocilia. To date, collective evidence indicates that two novel cadherins, Cadherin 23 (Cdh23) and Protocadherin 15 (Pcdh15), form the extracellular tip links between stereocilia and are thus critical members of the transduction machinery. Other members of the transduction complex are thought to include Harmonin, Myosin 1c, Myosin 15 and Whirlin. Although a number of candidates for the mechanotransduction channel have been put forth, the identity of the transduction channel in hair cells remains elusive. We hope to identify the components involved in hair cell function, particularly those required for mechanosensory transduction and synaptic transmission using forward genetics. By screening over 7000 genomes (Tuebingen Screens I & II), we have identified a total of 24 genes that are required for balance and hearing in zebrafish. These circler mutants are analogous to the mouse shaker-waltzer mutants and may help us to identify genes which are required for the function of mechanosensory organs such as the inner ear and lateral line system. We are also using reverse genetics to identify components of the hair cell transduction apparatus and to generate animal models for human hereditary deafness.


PUBLICATIONS
Pacentine, I.V., Nicolson, T. (2019) Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells. PLoS Genetics. 15:e1007635
Chandrasekhar, A., Guo, S., Masai, I., Nicolson, T., Wu, C.F. (2017) Zebrafish: from genes and neurons to circuits, behavior and disease. Journal of neurogenetics. 31:59-60
Nicolson, T. (2017) The genetics of hair-cell function in zebrafish. Journal of neurogenetics. 31(3):102-112
Sheets, L., He, X.J., Olt, J., Schreck, M., Petralia, R.S., Wang, Y.X., Zhang, Q., Beirl, A., Nicolson, T., Marcotti, W., Trapani, J.G., Kindt, K.S. (2017) Enlargement of ribbons in zebrafish hair cells increases calcium currents, but disrupts afferent spontaneous activity and timing of stimulus onset. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(26):6299-6313
Erickson, T., Morgan, C.P., Olt, J., Hardy, K., Busch-Nentwich, E.M., Maeda, R., Clemens-Grisham, R., Krey, J.F., Nechiporuk, A.V., Barr-Gillespie, P.G., Marcotti, W., Nicolson, T. (2017) Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt). eLIFE. 6
Maeda, R., Pacentine, I.V., Erickson, T., Nicolson, T. (2017) Functional analysis of the transmembrane and cytoplasmic domains of Pcdh15a in zebrafish hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(12):3231-3245
Erickson, T., Nicolson, T. (2016) Cell type-specific transcriptomic analysis by thiouracil tagging in zebrafish. Methods in cell biology. 135:309-28
Toro, C., Trapani, J.G., Pacentine, I., Maeda, R., Sheets, L., Mo, W., Nicolson, T. (2015) Dopamine Modulates the Activity of Sensory Hair Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(50):16494-16503
Nicolson, T. (2015) Ribbon Synapses in Zebrafish Hair Cells. Hearing Research. 330(Pt B):170-7
Erickson, T., Nicolson, T. (2015) Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish. BMC Genomics. 16:842
Sheets, L., Hagen, M.W., Nicolson, T. (2014) Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons. PLoS One. 9:e107256
Maeda, R., Kindt, K.S., Mo, W., Morgan, C.P., Erickson, T., Zhao, H., Clemens-Grisham, R., Barr-Gillespie, P.G., Nicolson, T. (2014) Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2. Proceedings of the National Academy of Sciences of the United States of America. 111(35):12907-12
Grisham, R.C., Kindt, K., Finger-Baier, K., Schmid, B., and Nicolson, T. (2013) Mutations in ap1b1 Cause Mistargeting of the Na(+)/K(+)-ATPase Pump in Sensory Hair Cells. PLoS One. 8(4):e60866
Kalueff, A.V., Gebhardt, M., Stewart, A.M., Cachat, J.M., Brimmer, M., Chawla, J.S., Craddock, C., Kyzar, E.J., Roth, A., Landsman, S., Gaikwad, S., Robinson, K., Baatrup, E., Tierney, K., Shamchuk, A., Norton, W., Miller, N., Nicolson, T., Braubach, O., Gilman, C.P., Pittman, J., Rosemberg, D.B., Gerlai, R., Echevarria, D., Lamb, E., Neuhauss, S.C., Weng, W., and Bally-Cuif, L. (2013) Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond. Zebrafish. 10(1):70-86
Sheets, L., Kindt, K.S., and Nicolson, T. (2012) Presynaptic CaV1.3 Channels Regulate Synaptic Ribbon Size and Are Required for Synaptic Maintenance in Sensory Hair Cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(48):17273-17286
Obholzer, N., Swinburne, I.A., Schwab, E., Nechiporuk, A.V., Nicolson, T., and Megason, S.G. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing. Development (Cambridge, England). 139(22):4280-4290
Kindt, K.S., Finch, G., and Nicolson, T. (2012) Kinocilia mediate mechanosensitivity in developing zebrafish hair cells. Developmental Cell. 23(2):329-341
Einhorn, Z., Trapani, J.G., Liu, Q., and Nicolson, T. (2012) Rabconnectin3alpha promotes stable activity of the H+ pump on synaptic vesicles in hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(32):11144-11156
Glover, G., Mueller, K.P., Söllner, C., Neuhauss, S.C., and Nicolson, T. (2012) The Usher gene cadherin 23 is expressed in the zebrafish brain and a subset of retinal amacrine cells. Molecular Vision. 18:2309-2322
Sheets, L., Trapani, J.G., Mo, W., Obholzer, N., and Nicolson, T. (2011) Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England). 138(7):1309-1319
Trapani, J.G., and Nicolson, T. (2011) Mechanism of spontaneous activity in afferent neurons of the zebrafish lateral-line organ. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(5):1614-1623
Mo, W., and Nicolson, T. (2011) Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapses. PLoS One. 6(11):e27146
Mo, W., Chen, F., Nechiporuk, A., and Nicolson, T. (2010) Quantification of vestibular-induced eye movements in zebrafish larvae. BMC Neuroscience. 11:110
Wright, M.A., Mo, W., Nicolson, T., and Ribera, A.B. (2010) In vivo evidence for transdifferentiation of peripheral neurons. Development (Cambridge, England). 137(18):3047-3056
Trapani, J.G., and Nicolson, T. (2010) Chapter 8 - Physiological recordings from zebrafish lateral-line hair cells and afferent neurons. Methods in cell biology. 100:219-231
Trapani, J.G., Obholzer, N., Mo, W., Brockerhoff, S.E., and Nicolson, T. (2009) Synaptojanin1 is required for temporal fidelity of synaptic transmission in hair cells. PLoS Genetics. 5(5):e1000480
Obholzer, N., Wolfson, S., Trapani, J.G., Mo, W., Nechiporuk, A., Busch-Nentwich, E., Seiler, C., Sidi, S., Söllner, C., Duncan, R.N., Boehland, A., and Nicolson, T. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(9):2110-2118
Geisler, R., Rauch, G.J., Geiger-Rudolph, S., Albrecht, A., van Bebber, F., Berger, A., Busch-Nentwich, E., Dahm, R., Dekens, M.P., Dooley, C., Elli, A.F.,Gehring, I., Geiger, H., Geisler, M., Glaser, S., Holley, S., Huber, M., Kerr, A., Kirn, A., Knirsch, M., Konantz, M., Kuchler, A.M., Maderspacher, F., Neuhauss, S.C., Nicolson, T., Ober, E.A., Praeg, E., Ray, R., Rentzsch, B., Rick, J.M., Rief, E., Schauerte, H.E., Schepp, C.P., Schonberger, U., Schonthaler, H.B., Seiler, C., Sidi, S., Söllner, C., Wehner, A., Weiler, C., Nüsslein-Volhard, C. (2007) Large-scale mapping of mutations affecting zebrafish development. BMC Genomics. 8(1):11
Söllner, C., and Nicolson, T. (2005) The Zebrafish as a Genetic Model to Study Otolith Formation. Biomineralization (2nd Edition). :229-242
Seiler, C., Finger-Baier, K.C., Rinner, O., Makhankov, Y.V., Schwarz, H., Neuhauss, S.C., and Nicolson, T. (2005) Duplicated genes with split functions: independent roles of protocadherin15 orthologues in zebrafish hearing and vision. Development (Cambridge, England). 132(3):615-623
Nicolson, T. (2005) The genetics of hearing and balance in zebrafish. Annual review of genetics. 39:9-22
Söllner, C., Schwarz, H., Geisler, R., and Nicolson, T. (2004) Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish. Development genes and evolution. 214(12):582-590
Seiler, C., Ben-David, O., Sidi, S., Hendrich, O., Rusch, A., Burnside, B., Avraham, K.B., and Nicolson, T. (2004) Myosin VI is required for structural integrity of the apical surface of sensory hair cells in zebrafish. Developmental Biology. 272(2):328-338
Paukert, M., Sidi, S., Russell, C., Siba, M., Wilson, S.W., Nicolson, T., and Grunder, S. (2004) A family of acid-sensing ion channels (ASICs) from the zebrafish: Widespread expression in the central nervous system suggests a conserved role in neuronal communication. The Journal of biological chemistry. 279(18):18783-18791
Söllner, C., Rauch, G-J., Siemens, J., Geisler, R., Schuster, S.C., The Tübingen 2000 Screen Consortium, Müller, U., and Nicolson, T. (2004) Mutations in cadherin 23 affect tip links in zebrafish sensory hair cells. Nature. 428(6986):955-959
Sidi, S., Busch-Nentwich, E., Friedrich, R., Schoenberger, U., and Nicolson, T. (2004) gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses. The Journal of neuroscience : the official journal of the Society for Neuroscience. 24(17):4213-4223
Busch-Nentwich, E., Söllner, C., Roehl, H., and Nicolson, T. (2004) The deafness gene dfna5 is crucial for ugdh expression and HA production in the developing ear in zebrafish. Development (Cambridge, England). 131(4):943-951
Söllner, C., Burghammer, M., Busch-Nentwich, E., Berger, J., Schwarz, H., Riekel, C., and Nicolson, T. (2003) Control of crystal size and lattice formation by starmaker in otolith biomineralization. Science (New York, N.Y.). 302(5643):282-286
Concha, M.L., Russell, C., Regan, J.C., Tawk, M., Sidi, S., Gilmour, D.T., Kapsimali, M., Sumoy, L., Goldstone, K., Amaya, E., Kimelman, D., Nicolson, T., Gründer, S., Gomperts, M., Clarke, J.D.W., and Wilson, S.W. (2003) Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron. 39(3):423-438
Sidi, S., Friedrich, R.W., and Nicolson, T. (2003) NompC TRP channel required for vertebrate sensory hair cell mechanotransduction. Science (New York, N.Y.). 301(5629):96-99
Ernest, S., Rauch, G.J., Haffter, P., Geisler, R., Petit, C., and Nicolson, T. (2000) Mariner is defective in myosin VIIA: a zebrafish model for human hereditary deafness. Human molecular genetics. 9(14):2189-2196
Nicolson, T., Ernest, S., Rauch, J., Petit, C., Söllner, C., Seiler, C., and Busch, E. (2000) Isolation of the genes responsible for hearing disorders in zebrafish. The European journal of neuroscience. 12:316
Seiler, C. and Nicolson, T. (1999) Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants. Journal of neurobiology. 41(3):424-434
Geisler, R., Rauch, G.J., Baier, H., van Bebber, F., Brobeta, L., Dekens, M.P., Finger, K., Fricke, C., Gates, M.A., Geiger, H., Geiger-Rudolph, S., Gilmour, D., Glaser, S., Gnugge, L., Habeck, H., Hingst, K., Holley, S., Keenan, J., Kirn, A., Knaut, H., Lashkari, D., Maderspacher, F., Martyn, U., Neuhauss, S., Neumann, C., Nicolson, T., Pelegri, F., Ray, R., Rick, J.M., Roehl, H., Roeser, T., Schauerte, H.E., Schier, A.F., Schönberger, U., Schönthaler, H.-B., Schulte-Merker, S., Seydler, C., Talbot, W.S., Weiler, C., Nüsslein-Volhard, C., and Haffter, P. (1999) A radiation hybrid map of the zebrafish genome. Nature Genetics. 23(1):86-89
Nicolson, T., Rüsch, A., Friedrich, R.W., Granato, M., Ruppersberg, J.P., and Nüsslein-Volhard, C. (1998) Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutants. Neuron. 20:271-283

NON-ZEBRAFISH PUBLICATIONS