Fig 2

Fig 2 IP₃R2 drives pigmentation in vivo.
(A) qRT-PCR analysis showing decrease in IP₃R2 mRNA expression after 48 h post fertilization (hpf) on zebrafish larvae injected with 400 μM IP₃R2 morpholino (N = 3). (B) Representative images of Assam wild type (ASWT) zebrafish after 48hpf injected with 400 μM IP₃R2 morpholino. (C) Bar graph showing melanin content estimation of zebrafish after 48hpf injected with 400 μM IP₃R2 morpholino (N = 3 with 20 embryos in each “N”). (D) qRT-PCR analysis showing increase in IP₃R2 mRNA expression after 48hpf on zebrafish larvae injected with 400 μM IP₃R2 morpholino and rescued with 120 ng of IP₃R2 mRNA (N = 4). (E) Representative images of ASWT zebrafish embryos after 48hpf injected with 400 μM IP₃R2 morpholino and rescued with 120 ng of IP₃R2 mRNA. (F) Bar graph showing melanin content estimation of zebrafish embryos after 48hpf injected with 400 μM IP₃R2 morpholino and rescued with 120 ng of IP₃R2 mRNA (N = 3 with 20 embryos in each “N”). Data presented are mean ± SEM. For statistical analysis, one-sample t test was performed for panels A, C, D, F using GraphPad Prism software. Here, * p < 0.05, ** p < 0.01 and **** p < 0.0001.