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Martinez-Mayer et al., 2026 - Generation of a prop1 knockin zebrafish enables single-cell transcriptomics of early pituitary development
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Fig. 1 Generation and expression pattern of a prop1:GAL4 knock-in line. (A) Scheme of the reporter generated by targeted insertion of a P2A-GAL4-VP16 sequence in exon 2 of prop1. (B) Whole-mount ISH against prop1 showing the pituitary invagination process from 24 hpf to 32 hpf. (C) The pituitary invagination process as seen by confocal microscopy using the prop1:GAL4 reporter in combination with the UAS:mScarlet-CAAX line. (D) Co-expression of prop1 mRNA as examined at 28 hpf via FISH (cyan) and immunofluorescence against mScarlet (purple) in (prop1:GAL4; UAS:mScarlet-CAAX) embryos.

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