ZFIN Image: Choi et al., 2026, Figure 5

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Figure Caption

Figure 5

3D interrogation of poorly recognized antigens using HyPer-3D

(A–F) Sections of the same testis were left as untreated controls (Cont) or treated with HyPer-3D (HyP), and then formamide/glycerol cleared after IF for the adult stem cell markers cKIT (A and B, green), STRA8 (C and D, green; inset shows high magnification, arrows highlight stained cells, and asterisks indicate regions of background noise), and GFR-α1 (E and F, red). Scale bars, 20 μm.

(G) SBRs (∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

(H) 3D imaging of testis tubules for MCAM (red) and GFR-α1 (green) enabled visualization of spermatogonial stem cells in A-paired spermatogonia (arrows) and A-aligned spermatogonia at 4-cell (arrow heads) or 8-cell (bracket) stages of differentiation.

Acknowledgments

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