IMAGE

Figure 4.

ID

ZDB-IMAGE-260423-59

Genes

EGFP

Antibodies

Publication

Hernandez et al., 2026 - Insulin Induces Outer Blood-Retinal Barrier Disruption via Downregulation of Claudin-19

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Figures for Hernandez et al., 2026

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Figure Caption

Figure 4.

Insulin causes claudin-19 breakdown in vivo. (A) Schematic of the EGFP–claudin-19 construct. (B) Generation of Tg(rpe65a:cldn19-EGFP) transgenic zebrafish. (C) Transgenic larvae at 122 hours post-fertilization showed the EGFP heart marker indicating transgenesis. (D) Immunofluorescence of claudin-19 expression in the RPE after 72 hours under different insulin conditions. Quantification is shown at the right, with data expressed as mean ± SD (n = 31–40 eyes). Scale bar: 50 µm. *P < 0.0051 (one-way ANOVA with Barlett's test). (E) Glucose uptake (glucose assay) and insulin levels (ELISA), shown as mean ± SD (n = 4). Scale bar: 50 µm. Glucose uptake: *P < 0.0015; **P < 0.0015 (one-way ANOVA with Brown–Forsythe test). Insulin levels: ****P < 0.0001 (ELISA). (F) Claudin-19 expression in larvae under control, glucose, insulin, and insulin plus glucose. Each dot represents one eye. Scale bar: 50 µm. *P < 0.0051; **P < 0.00150; ***P < 0.0004 (ordinary one-way ANOVA with Brown–Forsythe test). (G) Western blot analysis of 6 dpf larvae under control, glucose, insulin, and insulin plus glucose treatment. *P < 0.03; **P < 0.01 (one way ANOVA with Tukey's multiple comparison test).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.