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Figure 6.

ID: ZDB-IMAGE-260423-56
Publication: Wei et al., 2026 - A Novel CRYBB2 Splicing Mutation Is Associated With Lens Extracellular Matrix Remodeling and Vascular Alterations in Congenital Cataract
All Figures: Figures for Wei et al., 2026

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Figure Caption

Figure 6.

Transcription factor profiling and signaling pathway analysis in retinal vascular and lens-associated subclusters following crybb2 knockout. (A) Pseudotime trajectory of single-cell transcriptomes, colored by subcluster identity, showing developmental bifurcation in the Endothelium_Active population. (B) Pseudotime trajectory colored by experimental group, displaying distribution of WT and crybb2^+/− knockout cells. (C) Heatmap of the top 50 transcription factors in endothelial cells. Rows represent TFs, and columns represent groups (WT vs. crybb2^+/−). Expression values are scaled by row (scale = “row”). (D) BEAM branch heatmap of endothelial pseudotime trajectory, showing fate-biased gene modules identified by branch-dependent expression analysis. Genes are ordered by pseudotime and grouped by module identity.

Acknowledgments

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