IMAGE

Figure 4.

ID

ZDB-IMAGE-260423-54

Genes

EGFP

Antibodies

Ab8-kdrl

Publication

Wei et al., 2026 - A Novel CRYBB2 Splicing Mutation Is Associated With Lens Extracellular Matrix Remodeling and Vascular Alterations in Congenital Cataract

All Figures

Figures for Wei et al., 2026

Image

Figure Caption

Figure 4.

Crybb2 deficiency impairs hyaloid vessel development and permeability in zebrafish. (A) Confocal imaging of hyaloid vasculature in Tg(flk1:EGFP) zebrafish larvae at 5 dpf. White dashed lines outline vitreous vascular structures; boxed regions indicate magnified views. (B) Quantification of vascular density across experimental groups, analyzed using ImageJ. Each group included at least eight larvae (n ≥ 8). Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc multiple comparison test. Data are presented as mean ± SEM. (C) Assessment of tracer distribution by intravenous injection of TMR-MEP (red) at 5 dpf, imaged together with flk1:EGFP-positive vasculature (green). Scale bar: 50 µm. (D) Immunofluorescence staining for Flk-1 (VEGFR2/KDR, green) at 48 hpf. Nuclei were counterstained with DAPI (blue). Z-stack confocal images acquired at magnification ×60. Scale bar: 60 µm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.