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Fig. 2 Heart looping defects in tbx2a and tbx2b mutant zebrafish 48 hpf. A Schematic of CRISPR-Cas9 mutations of tbx2a and tbx2b. Each colored block indicates an exon for that respective gene. B Fluorescent imaging of GFP-labeled hearts from myl7: GFP transgenic embryos at 48 hpf. C Polar histograms displaying the distribution of heart looping angles in wild-type (WT), tbx2a mutant, and tbx2b mutant zebrafish at 48 h post-fertilization (hpf). Each bar represents the frequency of observed looping angles, color-coded by genotype: Wild Type (light green), Heterozygous (orange), and Homozygous (blue)
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