IMAGE

Fig. 8

ID

ZDB-IMAGE-260403-36

Genes

DsRed2
EGFP

Antibodies

Ab18-h3

Publication

Pérez-Escudero et al., 2026 - GPER1 reduces skin inflammation by inhibiting keratinocyte proliferation

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Figures for Pérez-Escudero et al., 2026

Image

Figure Caption

Fig. 8 Keratinocyte proliferation is essential for facilitating neutrophil infiltration into inflamed skin.

a Schematic of the experimental procedure used. Spint1a-deficient embryos were treated with Palbociclib or vehicle (DMSO) at 1 dpf by bath immersion. At 2 dpf, images were taken to analyze Nfkb reporter expression and neutrophil distribution. Anti-pH3 immunofluorescence was also performed. b Representative images of 2 dpf control and treated larvae, showing neutrophils and Nfkb reporter activity. c Quantification of Nfkb activity in skin in each group. Quantification of distribution (d) and number (e) of neutrophils in tail in every condition. e Quantification of neutrophil distribution in the tail in every condition. f Representative images of anti-pH3 immunofluorescence of 2 dpf control and treated larvae. g Number of pH3⁺ cells in skin in every condition. h Analysis of the correlation between the number of neutrophils and the number of pH3⁺ cells in skin. Each dot represents one individual, and the means and SEM for each group are also shown. P values were calculated by Student’s t test (c, d, f) and by Pearson Correlation test (g). ns, not significant; *P ≤ 0.05 ; ***P ≤ 0.001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Discov