Wild-type (WT) and mutant (MUT) AIP transcripts in WT, HET, and HMZ mutant aipwh239 larvae at 3 dpf (A, experiment 3a) and aipwh86 larvae at 5 dpf (B, experiment 3b). Transcript numbers were assessed using RNA-seq data. Data represent the number of reads that overlap the deletion site in the aip gene, averaged over 4 replicate samples. The numbers include both maternal and zygotic transcripts. Results show that HMZ mutant embryos have some WT maternal transcripts at 3 and 5 dpf. Mutant transcripts appear to be rapidly degraded, suggesting nonsense-mediated decay. Abbreviations used: WT: Wild-type; HET: Heterozygous; HMZ: Homozygous.
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