Figure 5

Figure 5

Whole-mount F-actin and nuclear staining reveals abnormal YSL distribution and premature actomyosin ring formation in zfPRL-3 morphants. Representative images of embryos injected with 4ng control morpholino (MO) or zfPRL-3 MO and stained for F-actin (phalloidin) and nuclei (DAPI; 4′,6-diamidino-2-phenylindole). Panels are grouped by staining modality to highlight different readouts: nuclei (DAPI) together with F-actin to provide positional context for YSL nuclei, versus F-actin–only staining to better resolve the actomyosin ring. Brackets in panels (A,B,E,F) indicate the region corresponding to the yolk syncytial layer (YSL). Arrowheads in panels (D,G,H) indicate the actomyosin ring. (A–D) Control morphants. (E–H) zfPRL-3 morphants. In zfPRL-3 morphants at ~60% epiboly, bracket in panel (F) highlight dispersed external YSL-associated nuclei, and arrowheads in panel (G) indicate the early appearance of the actomyosin ring relative to controls. Scale bars = 250 μm.