Figure 2

Figure 2

Expression of pancreatic cell and intestinal markers is decreased in zebrafish wrn^-/-

(A) Principal component analysis (PCA) of the wrn^+/+ and wrn^-/- individuals, color-coded by genotype.

(B) PCA of the wrn^+/+ individuals, colored by developmental stage.

(C) PCA of the wrn^-/- individuals, colored by developmental stage.

(D) PCA based on clustering, color-coded by cluster identity.

(E) PCA of the wrn^+/+ individuals, color-coded by cluster identity from Figure 2D.

(F) PCA of the wrn^-/- individuals, color-coded by cluster identity from Figure 2D.

(G) Gene filtering strategy used to identify 35 genes that were specifically downregulated in wrn^-/-.

(H) Heatmap of 35 genes downregulated in wrn^-/- at both 7–9 and 21–35 dpf.

(I) Tissue-specific expression of 32 genes (excluding cela1.3, cela1.5, and cela1.6) based on the Zebrafish Cell Landscape.

(J–M) Expression of pancreatic exocrine cell and intestinal markers in wrn^+/+ (black) and wrn^-/- (red). Exocrine cell markers: prss1 (J) and amy2a (K); Intestinal markers: fabp2 (L) and apoa1a (M). Statistical significance was assessed using Welch’s t test at each time point. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: not significant. The detailed p-values are shown in Table S5.