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Fig. 4

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ZDB-IMAGE-260215-174
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Figures for Chen et al., 2026
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Fig. 4

Hair cell staining of zebrafish after GJB2 c.109G > A mutation and knockdown. A. Specific fluorescence staining with 1 mM DASPEI (green) for 2 h, anesthetized with 0.03% MS-222, photographed under a 3× stereofluorescence microscope; B. Quantification of hair cell numbers in different groups of zebrafish using Image J, statistical analysis using GraphPad Prism 10. Blue arrows represent hair cells in the inner ear and red arrows represent hair cells in the lateral line system. n ≥ 10. Each column and bar represent the mean ± SEM. All data were statistically analyzed by one-way ANOVA, * p < 0.05 indicates a significant difference, and *** p < 0.001 indicates an extremely significant difference.

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