IMAGE

FIGURE 4

ID

ZDB-IMAGE-260129-39

Antibodies

Ab8-krt18

Publication

Han et al., 2026 - Multiple susceptibility enhancer variants increasing ADD3 expression predisposes to biliary atresia risk

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Figures for Han et al., 2026

Image

Figure Caption

FIGURE 4

Knockdown or overexpression of add3a induces biliary dysfunction in zebrafish. (A) Lateral view of 5 dpf zebrafish larvae after ingestion of PED-6. The gallbladders of larvae injected with add3a mRNA or MOs were smaller compared to control embryos. (B) Quantitative analysis showed that the accumulation of PED-6 in the gallbladders of mRNA-injected larvae and morphants were significantly reduced compared to control larvae. Scale bar = 100 μm. Chi-square test was used. *P < 0.05; **P < 0.01; ***P < 0.001. (C) Confocal projection of 5 dpf larvae stained for cytokeratin 18, comparing control larvae, mRNA-injected larvae, and add3a morphants. The intrahepatic bile ducts in both mRNA-injected larvae and add3a morphants appeared sparser than in controls. n = 10 for each group. (D) Confocal projection of 5 dpf larvae stained for cytokeratin 18, showing that the gallbladders of both mRNA-injected larvae and add3a morphants were smaller than those of controls. n = 10 for each group. Gallbladder cells are indicated by white dots.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Genet